Labeling reagent and labeling method for schizopygopsis younghusbandi
A technology of labeling reagents and labeling methods, applied in chemical instruments and methods, fish farming, luminescent materials, etc., to achieve the effect of small damage, no toxic side effects, and reduce the impact of fry
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[0028] Preparation of cactus extract: wash the fresh cactus with deionized water, mix the fresh cactus and water at a mass ratio of 1:10, and then extract it ultrasonically. The extraction temperature is 50°C, and the extraction time is 60 minutes; the extraction frequency is 75KHz, and the ultrasonic output power is 100W. ; After cooling, filter and centrifuge at 5000r / min to get the supernatant.
[0029] Prepare the labeling reagent for Lhasa naked schistos: the mass ratio of Alizarin Red-S and n-butylbenzisothiazolone is 1:5:1, the amount of cactus extract is 100mL per gram of fluorescent labeling mixture, mix Prepared labeling reagents.
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