High-stability nano-emulsion with photoprotection effect and preparation method of high-stability nano-emulsion
A nano-emulsion, high-stability technology, used in medical formulations containing active ingredients, skin care preparations, pharmaceutical formulations, etc. The problem is to overcome the low bioavailability, excellent moisturizing effect and simple preparation method.
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preparation example Construction
[0036] The preparation method of the nanoemulsion is as follows: mix water, moisturizing agent, skin conditioner, and pH regulator, heat to 70-80°C to dissolve to obtain phase A, and keep it warm for later use; phase B is pre-weighed or pre-dispersed for later use; agent, emulsifier, and solvent, heated to 80-85°C, homogenized for 3 minutes, cooled to 30°C or below, to obtain phase C, and set aside; weigh Echinacea purpurea extract, Mirabilis extract, Mix and stir the extracts of sage root and alfalfa evenly to obtain phase D, which is set aside; put phase B into phase A, homogenize for 3 minutes, control the temperature at 70-80°C, cool the obtained content to 40-45°C, Add phase C and phase D in turn, homogenize for 3 minutes, and mix well to obtain an emulsion.
[0037] In a specific embodiment, the components of the highly stable nanoemulsion with photoprotective efficacy are basically in the following ranges (parts and ratios are by weight):
[0038] 70-95 parts of phase ...
experiment example 1
[0058] Experimental Example 1: Immediate, 8h moisturizing test
[0059] Select the measurement area of the subject's forearm flexion side as 2×2cm 2 , apply 8 μL of the product (nano-emulsion, common emulsion prepared in Example 12) to the area to be tested, and pat and absorb it with a finger cot; use a Corneometer (CK, Germany) before use, 0h, 2h, 4h and 8h after use respectively. ) to measure the moisture content of the stratum corneum, measure 5 times at the same point, take the average value of 3 times and record after excluding the maximum and minimum values.
[0060] The experimental results are shown in Table 4. After comparing the nanoemulsion prepared in Example 12 of the present invention with the common emulsion, it is found that the nanoemulsion of the present invention has a very excellent instant moisturizing effect and can maintain a relatively high water content after 8 hours.
[0061] (Remarks: the rate of increase or decrease of moisture in the stratum co...
experiment example 2
[0064] Experimental Example 2: Determination of Reactive Oxygen Species (ROS)
[0065] In the human body, reactive oxygen species are continuously produced in keratinocytes and fibroblasts, and then quickly eliminated by non-enzymatic and enzymatic antioxidants. When the skin is exposed to external stimuli, such as ultraviolet radiation or blue light radiation, excessive active oxygen will be produced, and after a series of transformation processes, it will accelerate skin aging and endanger health. In this experiment, the molecular fluorescent probe 2',7'-dichlorodihydrofluorescein diethyl ester H 2 DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) was used to measure the inhibitory effect of the nanoemulsion prepared in the present invention on hydrogen peroxide-induced ROS in human dermal fibroblasts (HDFs). h 2 DCFDA itself has no fluorescence. After interacting with ROS in the cell, it can generate the fluorescent product dichlorofluorescein (DCF, 2',7'-Dichlorofluores...
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