Real-time fluorescent quantitative PCR detection reagent for glandular stomach necrosis virus and application
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A real-time fluorescent quantitative and necrotic virus technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of non-standardization of CPNV and achieve the effect of improving detection sensitivity
Pending Publication Date: 2021-06-01
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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[0003] In recent years, studies have found that chicken proventricular necrosis virus (CPNV) belongs to the double-stranded RNA virus family and the genus Birnacirus virus, and is closely related to infectious viral gastritis, and it is speculated that CPNV is the most important pathogen causing TVP. There is no standardized and precise solution for the detection of CPNV
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[0028]In order to facilitate understanding of the present invention, the present invention will be described more fully described with reference to the related drawings. The preferred embodiment of the present invention is given in the drawings. However, the present invention can be implemented in many different forms, and is not limited to the embodiments described herein. Conversely, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thoroughly.
[0029]All techniques and scientific terms used herein are identical to those skilled in the art, unless otherwise defined. The terms used herein in the specification of the present invention are intended to describe specific embodiments, and is not intended to limit the invention. The terms "and / or" as used herein include any and all combinations of one or more associated items.
[0030]As used herein, the term "target nucleic acid sequence", "target nucleic acid", and "tar...
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Abstract
The invention discloses a primer pair for detecting glandular stomach necrosis virus. The sequences of the primer pair are shown as SEQ ID NO. 1 and SEQ ID NO. 2. The invention also discloses a real-time fluorescent quantitative PCR detection kit for the glandular stomach necrosis virus. The real-time fluorescent quantitative PCR detection kit comprises the primer pair. The invention also discloses a non-diagnostic purpose detection method for the glandular stomach necrosis virus. Results prove that the primer pair and the corresponding method can specifically detect CPNV in a sample, compared with a traditional detection method, the method of the invention has the advantages that the detection sensitivity can be improved, the quantitative analysis effect can be achieved, and the method is particularly suitable for detecting the glandular stomach necrosis virus from chicken. The real-time fluorescent quantitative PCR detection method for the glandular stomach necrosis virus, which is established by the research, can fill the blank in the aspect of detection of the CPNV, and provides an extremely important technical support for accurate prevention and control of chicken infectious virus proventriculitis.
Description
Technical field[0001]The present invention relates to the technical field of biological genetic engineering, in particular, involving the real - time fluorescent quantitative PCR detection reagents and applications of adeno necrosis virus.Background technique[0002]Transmissible Viral Proventriculitis (TVP) is a chronic consumable digestive tract disease caused by viruses, which can lead to typical adenitis symptoms. The disease is more than chicks, which is mainly characterized by poor growth, slow growth, and feces are mainly characterized. Once the chicken is onset, the initial stage of onset is the shortage of turning, the feathers are fluffy, the spirit is depressed, picky food, the middle and late, the diseased chicken is thin, diarrhea, diof doped ungarized feed; severe chicken The whole body is pale, final failure. Cabinet visible adenophyte gastroponics, milk, touches, hard, adrenalous wall edema, thickening, adrenal nipple swelling, bleeding, ulcer, etc., pathological secti...
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