A device for radioactive experimentation of breast cancer cell culture in vitro

Abstract

The invention discloses a radioactive experiment device for culturing breast cancer cells in vitro, and belongs to the technical field of radiobiological experiment equipment. The device includes a device body and a cover. There is a supporting plate in the receiving groove of the device body. The supporting plate is connected to the device body through a slow down device. The culture plate is more stable when it is put in. The drainage port and water storage tank are set on the culture plate, and the culture holes on the culture plate are evenly arranged in the water storage tank, which can realize the irradiation of multiple groups of cells in the same experimental environment; there are three panels on the supporting plate. , a limit hole is set on the side of the gap around the three panels of the supporting plate, a supporting frame is fixed under the supporting plate, and a limiting mechanism is installed on the supporting frame, which can realize the fixed position of the culture plate; the device body is provided with a water inlet and a water outlet, A one-way valve is provided in the water inlet, and sealing plugs are connected to the water inlet and the water outlet to ensure that the cells in each culture well are uniformly irradiated.

Description

Technical field: [0001] The invention relates to a device for radiation biology experiments, in particular to a device for radiation experiments of breast cancer cells cultured in vitro. Background technique: [0002] Breast cancer is one of the common malignant tumors in women. One of its characteristics is that the disease develops rapidly, and it is easy to develop rapidly from the early stage to the late stage. Now, radiotherapy, surgery, chemotherapy, especially neoadjuvant chemotherapy are often used in combination in clinical practice. Timely and feasible radiotherapy for patients diagnosed with locally advanced breast cancer can control the progression of the disease and facilitate the development of various subsequent treatments. With the continuous research on the pathogenesis of breast cancer, the significance of targeting genes to improve the radiosensitivity of tumor cells for the treatment of tumors has reached a new level. Taking breast cancer cell line as a...

AI Technical Summary

Problems solved by technology

In order to ensure that the control objects in different culture wells have the same irradiation depth, an equal amount of culture solution is added to each culture well. In practice, it is difficult to ensure that the amount of culture solution is uniform.

It is easy to shake or overturn when the petri dish is transferred to the irradiation table, and there is a possibility of sliding and shifting during irradiation

When multiple cell culture dishes are required for irradiation, the controllability of the environment around two adjacent culture dishes is poor, and it is easy to produce uneven irradiation and different irradiation results

In addition, in the existing experiments, they are often directly exposed to the external environment for many times, and the living environment of the cells fluctuates greatly.

The above deficiencies may lead to large experimental errors

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