Solid composition for plant care containing fungal sclerotium and use thereof
A solid composition, sclerotia technology, applied in the direction of chemicals for biological control, microorganism-based methods, fungi, etc., can solve the problems of high hidden danger of chemical pollution, less than 11 days of survival time, limited application, etc., To maximize the control effect, prolong the supply time, provide convenience and economy
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[0054] implementation preparation
[0055] The fungi used in the present invention can be obtained from sclerotia of phytopathogenic fungi among fungi reported to be capable of forming sclerotia (Smith ME, Henkel TW., Rollins JA. 2015. How many fungi make scleortia? Fungal Ecology 13, 211-220) constitute.
[0056] Among the sclerotium-forming fungi, particularly, sclerotia obtained from one or more fungi belonging to the genus Sclerotinia, Sclerotinia, and Rhizoctonia can be used, and the sclerotia composition can also contain sclerotia The carbon source, nitrogen source, etc. or nutrients containing trace elements required for germination and development of the plant can be designed in different ways according to different target plants.
[0057] Preferably, the water-absorbing material used in the sclerotia composition may have the property of absorbing water more than 10 times its own weight, thereby providing the effect of prolonging the water supply time, so that nutrien...
Embodiment 1
[0060] Embodiment 1. The viability comparison of sclerotia in dry environment
[0061] In a dry environment, compare the viability of mycelial cultures and sclerotia. The sclerotium (hereinafter referred to as "sclerotia 1") of Sclerotinia trifolii BWC98-105 (Accession No.: KACC93004P, hereinafter referred to as "sclerotia 1") which is a sclerotia-forming bacterium and Sclerotinia delphinium Sclerotia (hereinafter referred to as "sclerotia 2") of BWC04-107 (Accession No.: KACC93031P, hereinafter referred to as "sclerotia-forming bacteria 2"), a mycelial culture in the state of hardened sclerotia itself and mycelium culture Compare.
[0062] The sclerotia of sclerotia-forming bacteria were ensured by culturing on potato dextrose agar medium (potato dextrose agar, PDA; Difco) at a temperature of 25°C for more than 2 weeks. Substratum culture was carried out for 5 days to ensure the mycelial culture of sclerotia-forming bacteria at the stage before sclerotia formation.
[0063] ...
preparation example 1 to preparation example 7
[0069] Preparation Example 1 to Preparation Example 7. Preparation of Sclerotia Composition
[0070] Based on the above-mentioned Example 1, the solid composition was completed with the composition ratio shown in Table 2. First, put the prepared sclerotium into the sugar coating machine, add the powder of the remaining composition while rotating, spray water appropriately to coat the sclerotia, and repeat the process of adding powder and spraying water again for coating , to prepare spherical particles with a diameter of about 4 mm and dry, thereby completing the composition of the preparation example. The composition ratios shown in Table 2 are composition ratios excluding moisture.
[0071] In the composition, commercially available glucose powder was used as a carbon source, defatted soybean meal powder was used as a nitrogen source, and citric acid powder was used as a preservative. When formulating granules, sodium alginate powder was used as a water-absorbing material ...
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