Application of lncRNA in the diagnosis and treatment of recurrent miscarriage

A technology for recurrent miscarriage and products, applied in the fields of herbal medicine and molecular biology, can solve the problem of less lncRNA and achieve the effect of cycle promotion

A technology for recurrent miscarriage and products, applied in the fields of herbal medicine and molecular biology, can solve the problem of less lncRNA and achieve the effect of cycle promotion

CN112961913BActive Publication Date: 2021-10-22THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Application of lncRNA in the diagnosis and treatment of recurrent miscarriage
  • Application of lncRNA in the diagnosis and treatment of recurrent miscarriage
  • Application of lncRNA in the diagnosis and treatment of recurrent miscarriage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0179]1. Experimental method

[0180] 1. Culture of trophoblast cell lines

[0181] The trophoblast cell lines HTR-8 / SVneo (HTR8), JAR and HEK293T cells were cultured in DMEM / F12 medium, RPMI-1640 medium and DMEM medium containing 10% FBS, respectively, at 37°C and 5% CO 2 environment in a cell culture incubator. According to the growth rate of different cells, subculture once every 1-2 days. Record the doubling time of each cell line and draw the doubling curve.

[0182] 2. Collection of clinical specimens

[0183] From September 2017 to December 2019, 25 cases of villi tissue samples (6-10 weeks) of recurrent abortion patients and 23 cases (6-10 weeks) of normal pregnancy were obtained from the Second Hospital of Shandong University. The inclusion criteria for patients with recurrent miscarriage were 2 or more consecutive miscarriages, and clear causes such as genetic defects, infection, gynecological inflammation, and tumors were excluded. The total RNA, protein and ti...

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Abstract

The invention provides the application of lncRNA in the diagnosis and treatment of recurrent miscarriage, and belongs to the technical fields of herbal medicine and molecular biology. The present invention found for the first time that the expression level of LINC01088 is closely related to recurrent miscarriage, and its sensitivity and specificity in the diagnosis of recurrent miscarriage are high. Further studies have shown that LINC01088 can inhibit the proliferation and invasion of trophoblast cells, and inhibit the cycle and promote the apoptosis of trophoblasts, leading to miscarriage. Therefore, lncRNAs such as LINC01088, as predictive diagnostic markers and potential therapeutic targets for recurrent miscarriage, play an important role in the diagnosis and treatment of recurrent miscarriage, so they have good practical application value.

Description

technical field [0001] The invention belongs to the technical field of crude drug medicine and molecular biology, and specifically relates to the application of lncRNA in the diagnosis and treatment of recurrent miscarriage. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] According to the American Society for Reproductive Medicine, recurrent spontaneous abortion (RSA) refers to two or more consecutive embryo losses. In addition to genetic factors, abnormal uterine anatomy, infection and other clear causes, there are also recurrent miscarriages caused by unknown reasons. Due to the lack of effective clinical treatment measures, RSA has become one of the important causes o...

Claims

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Application Information

Patent Timeline
22 Oct 2021
Publication
CN112961913B
IPC
C12Q1/6883; A61K45/00; A61P15/06
CPC
A61K45/00; A61P15/06; C12Q1/6883; C12Q2600/136; C12Q2600/158; C12Q2600/178
Inventors
毛海婷; 赵汇