Fluorescence excitation block light source and fluorescence microscope

Abstract

The embodiment of the invention discloses a fluorescence excitation block light source and a fluorescence microscope. The light source comprises a light source identification driving module and at least one fluorescence excitation module, the fluorescence excitation module comprises a light-emitting element and a condensing lens, and the light-emitting element is used for emitting an excitation light beam; the condensing lens is used for condensing the excitation light beam to form a condensed light beam; the condensed light beam is used for exciting the to-be-detected sample to generate fluorescence; the fluorescence excitation module further comprises a shell, the light-emitting element is arranged at a first light transmitting opening, and the condensing lens is arranged in the shell. The excitation light beam enters the shell through the first light transmitting opening, the fluorescence condensation light beam enters a to-be-detected sample through a second light transmitting opening, and the fluorescence light beam carrying the characteristics of the to-be-detected sample is emitted through the second light transmitting opening and a third light transmitting opening in sequence; the light source identification driving module is electrically connected with the light-emitting element and used for controlling the light-emitting element to emit excitation light beams. The problems that in the prior art, a fluorescent light source is large in size, high in cost, large in optical loss and inconvenient to adjust optical power are solved.

Description

technical field

[0001] Embodiments of the present invention relate to the technical field of fluorescence imaging, and in particular to a fluorescence excitation block light source and a fluorescence microscope. Background technique

[0002] The excitation light source components of traditional fluorescence microscopes are mainly installed in the lampshade at the rear end of the microscope. The excitation light source components mainly include mercury lamps, xenon lamps, halogen lamps and LED light sources. Whether it is mercury lamps, xenon lamps, halogen lamps and other broad-spectrum light sources, or multiple single-color LED integrated multi-wavelength light sources, they are all installed behind the microscope. At the end, since these four types of light sources are not point light sources, it is difficult to collimate and concentrate the light emitted by the light source; and the light source is far away from the sample, and the loss in the light propagation process i...

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