An immune polypeptide and its application

An immune peptide and immune drug technology, applied in the field of biomedicine to achieve the effect of enhancing the body's immune response

Active Publication Date: 2022-08-05
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no relevant research on the use of a single drug that has the triple function of IDO1 inhibitor, PD1 / PD-L1 antagonist, and activation and enhancement of immune response for the preparation of immunotherapy drugs for tumors

Method used

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  • An immune polypeptide and its application
  • An immune polypeptide and its application
  • An immune polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Synthesis of P-peptide Indoximod-Gffy

[0084] The FMOC-short peptide solid phase synthesis method is used. The specific steps are:

[0085] 1) Take 0.5mmol 2-CL-TRT resin in the solid phase synthetic, add 10ml of water-free dichlorobial (DCM), and shake it on the shaking bed for 10 minutes to make the 2-CL-TRT resin full of swelling;

[0086] 2) Use an ear washing ball to press DCM from a solid phase synthesizer equipped with 2-CL-TRT resin to remove clean;

[0087] 3) Soluble the amino acids (FMOC-TYR (TBU)) protected by 0.5mmol FMOC in 10ml of water-free DCM, add 1mmol DIEPA, and then transfer to the above-mentioned solid phase synthesizer at room temperature for 1h;

[0088] 4) Closure: Use the ear washing ball to remove the reaction solution in the solid synthesizer, and then wash it with 10ml waterless DCM, 1min each time, washed 5 times a total of 5 times. 17: 1: 2 solution 20ml, reacts for 20min at room temperature;

[0089] 5) Use the ear washing ball to ...

Embodiment 2

[0095] Example 2 peptide Indoximod-G D F D F D Y's synthesis

[0096] Synthesis according to the FMOC-short peptide solid phase synthesis method of Example 1. Get the polypeptide Indoximod-G D F D F D Y. The polypeptide obtained by the Example 2 of the Example 2 is detected by the high -efficiency liquid color spectrum. figure 2 The structure is

[0097]

Embodiment 3

[0098] Example 3 peptide INDOXIMOD-G D F D F D Y- D N D Y D S D K D P D T D D D R D Q D Y D H D F

[0099] Synthesis according to the FMOC-short peptide solid phase synthesis method of Example 1. The combination of heterogeneity and cancer cell surface PD-L1 protein D PPA-1 sequence is listed as D N D Y D S D K D P D T D D D R D Q D Y D H D F, so the polypeptide applied for this application is referred to as INDOXIMOD-G D F D F D Y- D PPA-1. The polypeptide obtained by the Example 3 of the Example 3 is detected by the high -efficiency liquid color spectrum. image 3 The structure of the embodiment 3 polypeptide is

[0100]

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Abstract

The present invention relates to the technical field of biomedicine. The invention provides an immune polypeptide and its application, which are obtained by connecting an end capping group and a short peptide, wherein the short peptide is GFFY, G D F D F D Y and D One or more of PPA-1. The immune polypeptide can self-assemble to form supramolecular nanomedicine. The supramolecular nanomedicine can simultaneously reverse the immunosuppressive effect mediated by the accumulation of metabolites such as excessive tryptophan and kynurenine in the IDO pathway after entering the tumor microenvironment; The combination of L1 blocks the immune escape of tumor cells; at the same time, it activates and enhances the immune response of the body. The three parts work in parallel, so that the body can produce a strong immune response against the tumor to achieve the best effect in the immunotherapy of cancer. Therapeutic nanomedicine provides new ideas.

Description

Technical field [0001] The invention involves the field of biomedical technology, especially an immune polypeptide and its application. Background technique [0002] Tumor immunotherapy mainly controls the body's own immune system to restore the inhibitory immune response, block immune escape or enhance its own anti -tumor immunity to control and kill tumor cells. Among the many immunotherapy, immune examination points have shined. While obtaining a significant effect on the immune checkpoint inhibitors for PD-1 / PD-L1, the study of small molecular inhibitors such as shortptide antagonists also increased. With the continuous deepening of research, more immune examination points in tumor immunochemicals are found. Studies have shown that oltyramine-2, 3-bipotransomerase 1 (IDO1) is a key speed limit enzyme for human catalytic essential amino acids to be metabolized by canineurine. Immunosy enzymes related to the body's immunosuppressive effect. The increase in TRP exhaustion and KY...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/103C07K7/06C07K7/08C07K19/00A61K38/07A61K38/08A61K38/10A61P35/00B82Y5/00
CPCC07K5/1008C07K7/06C07K7/08A61P35/00B82Y5/00C07K2319/00A61K38/00
Inventor 杨志谋王玲李欣欣
Owner NANKAI UNIV
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