Compositions for treating oxidative stress-associated skin diseases and skin ageing and diagnosis of oxidative stress-associated skin diseases
A technology for oxidative stress and skin aging, used in medicine and beauty, to solve problems such as reducing the body's ability to repair itself
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[0342] Listing 1. Cosmetic use of a composition comprising a live P. acnes strain secreting RoxP (radical oxygenase of P. acnes) for preventing or reducing skin aging in a subject.
[0343] Listing 2. Composition for use in a method of preventing or treating oxidative stress-related skin diseases comprising a live RoxP-secreting P. acnes strain.
[0344] List 3. The composition for use according to List 2, wherein the oxidative stress-related skin disease is selected from the group comprising: actinic keratosis (AK), basal cell carcinoma (BCC), squamous cell carcinoma ( SCC), dandruff, seborrheic dermatitis, acne, inflammation, dermatitis, psoriasis, eczema, rosacea, urticaria, and vitiligo.
[0345] List 4. The use according to list 1 or the composition for use according to list 2 or 3, wherein the P. acnes strain endogenously secretes RoxP.
[0346] List 5. The use according to list 1 or 4 or the composition for use according to any one of lists 2 to 4, wherein the P. acnes...
Embodiment 1
[0421] Example 1: Expression of roxP depends on P. acnes phylotype
[0422] The rate of roxP expression was determined by real-time quantitative PCR (qPCR) in all major P. acnes phylotypes. P. acnes isolates were identified as phylotype I showing significantly higher expression levels of roxP compared to most phylotype II or III strains ( figure 1 ). Table 2 below provides the figure 1 The cohorts of strains used in the experiments are potentially shown in . Fold changes represent values obtained normalized to gapdh and related to roxP expression observed for P. acnes strain 266.
[0423] Table 2: In figure 1 The cohorts of strains used in the experiments are potentially shown in .
[0424]
[0425] Sequencing of each promoter region revealed the presence of 8 major sequences in the upstream region of RoxP ( Figure 9 ) and further showed a base substitution in the type II strain that affected the predicted -35 region of the roxP promoter, potentially enabling adver...
Embodiment 2
[0427] Example 2: RoxP homologues show comparable antioxidant activity
[0428] Although RoxP is highly conserved, with 99-100% amino acid identity among the majority of isolates (approximately 93%), subtypes of strains classified as P. acnes phylotypes II and III (approximately 7%) express A more distantly related protein, which has approximately 83% amino acid identity ( Figure 10 ). The reducing activity of different RoxP homologues was compared. The main variant (Type IB) and the less common homologue (Type II) from P. acnes AD24 medium could be successfully isolated from P. acnes KPA171202 medium. The latter was purified using macroporous gels imprinted with recombinant RoxP ( Figure 11 ), followed by verification of protein identity by immunoblot analysis (data not shown). Both congeners were able to reduce the cationic ABTS with equal efficiency as shown by the decrease in absorbance at 734 nm + substrates, and in doing so showed highly similar antioxidant activi...
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