Control device in purification amplification reaction based on PCR

Abstract

The invention relates to a control device in a purification amplification reaction based on PCR. According to a method, a reactant to be amplified, a freeze-drying ball and memory metal are contained in an amplification bin; when the reactant to be amplified enters the amplification bin, a first length and a second length of the memory metal at a first temperature and a second temperature are recorded; a first disturbance function T1 (t1i, L1i and Q1i) and a second disturbance function T2 (t2i, L2i and Q2i) are established, t1i and t2i represent the first temperature and the second temperature in the ith period respectively, and L1i and L2i represent the length of the corresponding memory metal in the ith period respectively; and Q1i and Q2i respectively represent the mixing degree of the reactant to be amplified and the freeze-dried ball in the ith period. The memory metal is arranged in the amplification bin, so that the related chemical reaction is more uniform under the action of disturbance, and the reaction is more sufficient.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a temperature control method based on a PCR amplification chamber. Background technique [0002] In the gene chip detection process, temperature control is the decisive factor in the PCR (Polymerase Chain Reaction, polymerase chain reaction) amplification process, and it is the key technology that determines whether the fluorescent signal can be seen. [0003] Temperature control usually includes three stages of temperature control, which are high temperature (92°C-96°C), medium temperature (69°C-73°C) and low temperature (52°C-56°C). In a complete PCR amplification process, the gene chip will be denatured in the high temperature stage, then annealed in the low temperature stage, and finally extended in the middle temperature stage. Completing the above three stages completes a PCR amplification. Generally, multiple above-mentioned processes are required to reach the predet...

AI Technical Summary

Problems solved by technology

However, the existing temperature monitoring methods are not very sensitive to temperature control and adjustment, which will have a certain impact on the PCR reaction, thus affecting the final fluorescence detection results.

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