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A diagnostic kit for lung cancer based on gene methylation in lung tissue

A lung cancer diagnosis and methylation technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial measurement/testing, etc., to achieve good application value

Active Publication Date: 2022-05-27
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The relationship between the methylation status of the aforementioned six genes and lung cancer has not been reported

Method used

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  • A diagnostic kit for lung cancer based on gene methylation in lung tissue
  • A diagnostic kit for lung cancer based on gene methylation in lung tissue
  • A diagnostic kit for lung cancer based on gene methylation in lung tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Bisulfite sequencing kit for lung cancer diagnosis

[0031] 1. Composition of the kit

[0032] The sample used in the kit is lung tissue DNA, and the reagents include bisulfite reagent, sequencing library building reagent and PCR amplification reagent.

[0033] The bisulfite reagent is a bisulfite reagent commonly used in the field for methylation sequencing, and its function is to convert unmethylated C bases into U, and the reagents are commercially available (for example: EpiTech Reagent). Sulfite Kit (A-59104), Tiangen DNA Bisulfite Conversion Kit (DP215), etc.).

[0034] The sequencing library building reagent is a reagent for performing end repair on DNA and adding sequencing adapters, and is commercially available, such as Ironman library building kit.

[0035]The PCR amplification reagents include primers, commercially available PCR master mixes for amplifying DNA segments of interest (CYCS, LOC283585, ZNF316 genes, THEG5, MIR1233-1 and LOC102724050)...

Embodiment 2

[0044] Embodiment 2 The methylation status of the aforementioned 6 genes is correlated with lung cancer

[0045] 1. Clinical samples

[0046] Patients with pulmonary nodules who were to undergo surgical resection were consecutively included, and the inclusion criteria were as follows: (1) pulmonary nodules were present on chest CT; (2) surgical resection was performed at the Department of Thoracic Surgery, West China Hospital, Sichuan University and confirmed by pathology (with sufficient sample size); (3) No previous history of lung cancer or other tumors; ④ No history of radiotherapy, chemotherapy, etc.; The exclusion criteria were as follows: ① Combined with imaging manifestations such as atelectasis, pneumonia, hilar enlargement or pleural effusion; ② Combined with other serious diseases (severe cardiovascular or pulmonary disease, etc.); a total of 100 cases of pulmonary nodules were included The specific clinical characteristics of the patients are shown in the table be...

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Abstract

The invention discloses a lung cancer diagnosis kit based on gene methylation in lung tissue, belonging to the field of tumor diagnosis reagents. The kit of the present invention includes reagents for detecting the methylation of any 1-6 genes in CYCS, LOC283585, ZNF316, THEG5, MIR1233-1, and LOC102724050, and can realize rapid diagnosis of lung cancer with high accuracy and good application prospects.

Description

technical field [0001] The invention belongs to the field of tumor diagnostic reagents. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest growing morbidity and mortality, and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men are the first among all malignant tumors, and the incidence and mortality of women are the second. [0003] Early prediction of the risk of lung cancer and early screening are of great significance for improving the prognosis of lung cancer. Through tumor markers, lung cancer risk prediction and early screening can be realized quickly and conveniently. After long-term research, there are many lung cancer markers, mainly including the following types: (1) autoantibodies; (2) other proteins other than autoantibodies; (3) metabo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2531/113C12Q2523/125C12Q2535/122C12Q2533/101C12Q2537/165
Inventor 李镭李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV