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Application of New Wenshen Shengjing Decoction (NWSSJD) as medicine for regulating expression level of H3K4me3

A technology of expression level and warming the kidney, which is applied in drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of undiscovered use of new Wenshen Shengjing drink, undiscovered relationship between H3K4me3 expression levels, etc., to achieve The effect of increasing the embryonic development rate and reducing the developmental rate

Pending Publication Date: 2021-08-06
JINLIN MEDICAL COLLEGE
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Problems solved by technology

[0004] However, the existing technology only uses Xin Wenshen Shengjing Decoction to treat sexual dysfunction and increase the incidence and maturation rate of sperm, but has not found the relationship between it and the expression level of H3K4me3, nor has it found that Xin Wenshen Shengjing Decoction Use in regulating H3K4me3 expression level

Method used

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  • Application of New Wenshen Shengjing Decoction (NWSSJD) as medicine for regulating expression level of H3K4me3
  • Application of New Wenshen Shengjing Decoction (NWSSJD) as medicine for regulating expression level of H3K4me3
  • Application of New Wenshen Shengjing Decoction (NWSSJD) as medicine for regulating expression level of H3K4me3

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Embodiment Construction

[0039] In order to enable those skilled in the art to better understand that the technical solutions of the present invention can be implemented, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0040] The methods used in the following examples are conventional methods unless otherwise specified, and all reagents used are commercially available.

[0041] 1 Materials and processing methods

[0042] 1.1 Test animals

[0043] Sexually mature Kunming strain 10-week-old male mice and 8-week-old female mice were used in the experiment. Mice lived in a temperature-controlled room with a 12h light / 12h dark photoperiod. Mice had free access to water and food. The treatment of mice was approved by the Ethics Committee of Jilin Medical University.

[0044] 1.2 Preparation of Xinwenshen Shengjing Drink

[0045] The compound Chinese medicine New Wenshen Shengjing Decoction (NWSSJD) contains ginseng 6g, Cynomori...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to application of a New Wenshen Shengjing Decoction (NWSSJD) to promotion of early embryonic development by maintaining the low level of H3K4me3 in sperms. A sexually mature male mouse is molded with cyclophosphamide, and drenches NWSSJD for 30 days, the treated male mouse is mated with a super-excreted female mouse, the developmental rate of embryos is counted in each stage, the expression of H3K4me3 in sperms and the embryos in each stage is detected by using an immunofluorescence staining method, the expression of methylase SETD1B in blastocysts is detected by using Western blotting, and the expression of development-related genes (OCT-4, NANOG and CDX2) and apoptosis-related genes (BCL-2 and P53) in the blastocyst is analyzed through qRT-PCR, and the result is analyzed and shows that the NWSSJD can promote the development of the early embryo by maintaining the normal H3K4me3 modification level in the sperm and the early embryo and inhibiting the apoptosis of embryonic cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of Xin Wenshen Shengjing Decoction as a medicine for regulating the expression level of H3K4me3. Background technique [0002] Xin Wenshen Shengjing Decoction is a traditional compound Chinese medicine, which is widely used in the treatment of male spermatogenesis disorders. New Wenshen Shengjing Decoction (New Wenshen Shengjing Decoction, NWSSJD) is composed of 15 kinds of traditional Chinese medicines, mainly including ginseng, antler, Cynomorium, astragalus, epimedium and angelica. It can maintain high androgen concentration in testicular tissue, promote the development of germ cells at all levels, inhibit the apoptosis of germ cells, and increase the incidence and maturity of sperm. An important criterion for testing drugs for the treatment of male spermatogenic disorders is whether they can produce normal offspring. Therefore, studying the effects o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61P15/08A61K35/32
CPCA61K36/258A61K36/185A61K36/481A61K36/296A61K35/32A61K36/64A61K36/232A61K36/8945A61K36/284A61K36/236A61K36/71A61K36/65A61K36/54A61K36/285A61K36/235A61P15/08
Inventor 潘晓燕雷钧涛孙艳美张万生王雪楠
Owner JINLIN MEDICAL COLLEGE
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