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Nucleic acid composition, kit and detection method for detecting methylation of breast cancer related genes

A technology of nucleic acid composition and methylation, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as accurate results, achieve sensitivity and specificity, improve quality of life, and save society resource effect

Active Publication Date: 2021-08-06
杭州圣庭医疗科技有限公司
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Problems solved by technology

[0009] At present, it is still impossible to find a nucleic acid composition and kit with accurate results, fast timeliness, high detection throughput, good specificity, and good sensitivity for early detection of breast cancer methylation. The present invention solves such problems and can indirectly Provide effective information for early diagnosis of human breast cancer, provide a simple and easy method for early detection, early treatment and monitoring of treatment effect

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  • Nucleic acid composition, kit and detection method for detecting methylation of breast cancer related genes
  • Nucleic acid composition, kit and detection method for detecting methylation of breast cancer related genes
  • Nucleic acid composition, kit and detection method for detecting methylation of breast cancer related genes

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Embodiment Construction

[0094] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0095] A method for detecting the methylation of breast cancer-related genes, comprising the steps of:

[0096] Step 1: extract the DNA of the sample to be tested, and use the converted DNA as a genomic DNA sample for PCR template;

[0097] The reagent used in the conversion treatment is bisulfite or bisulfite, and the sequence after bisulfite treatment is used for design, which can ensure the quality control of the sample by the housekeeping gene; the examples here are not exhaustive, as long as they can be used for All sequences subjected to pretreatment are applicable to the present invention.

[0098] Step 2: Perform PCR amplification on the genomic DNA sample using a kit for detecting methylation of breast cancer-related genes; as a preferred method, the reaction program for PCR amplification is: 95°C, 10min, 45cycles 95°C, 15sec, ...

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Abstract

The invention discloses a nucleic acid composition, a kit and a detection method for detecting methylation of breast cancer related genes. The nucleic acid composition comprises a methylation specific primer and a probe of a target site of an RASSF1A gene; a methylation specific primer and a probe of a target site of the APC gene; a methylation specific primer and a probe of a target site of the GSTP1 gene; a methylation specific primer and a probe of a CDH1 gene target site; and a specific primer and a probe of an ACTB reference gene; The kit prepared from the nucleic acid composition disclosed by the invention has the advantages of accurate methylation result of breast cancer related genes, rapidness, high throughput, simplicity in operation, high accuracy and sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a nucleic acid composition, a kit and a detection method for detecting methylation of breast cancer related genes. Background technique [0002] In 2020, 19.3 million people will be diagnosed with cancer, and 10 million people will die of cancer. Currently, one in five people worldwide will develop cancer in their lifetime. Breast cancer has become the most common cancer among women worldwide, accounting for 11.7% of new cancer cases. The global incidence of breast cancer has been on the rise since the late 1970s. Around the world, every 26 seconds, a woman is diagnosed with breast cancer; 2 million women suffer from breast cancer every year; almost every minute, a woman dies of breast cancer; 500,000 women die of breast cancer every year. There are about 300,000 new cases of breast cancer in my country every year. In the United States, breast tumors have a highe...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2600/166C12Q2531/113C12Q2545/101C12Q2545/113C12Q2523/125C12Q2563/107Y02A50/30
Inventor 谷红仓钱飞箭王云飞车仙荣
Owner 杭州圣庭医疗科技有限公司
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