A Method for Marking and Release of Silver Carp and Evaluation of Release Effect
An evaluation method and labeling technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of huge promotion value
Active Publication Date: 2022-06-17
XIAN UNIV OF TECH
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Problems solved by technology
However, there is no report on how effective silver carp release can be. There is an urgent need for an effective method to evaluate the effect of artificial release of silver carp.
Method used
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Embodiment
[0040] DNA extraction: Take silver carp tail fin ray tissue less than 0.5g, place it in a 1.5mL Eppendorf tube, label the number, and successively add 450ul STE DNA extraction buffer (10mmol / L Tris-HCl, pH8.0; 1mmol / L EDTA, pH8.0), 35ul SDS (10%), and finally 15ul proteinase K (0.2%) was added and mixed by inversion. Open the water bath in advance, add an appropriate amount of water, set the temperature to 55 °C, and put the mixed solution into 55 °C after the water temperature is constant. In the water bath, take out and invert and mix once every 30min until the solution is clear and transparent, which takes about 2h. Take it out, add 1 ul of RnaseA to each tube, and place it in a water bath at 37°C for 1 hour. Take out the Eppendorf tube, add an equal volume (about 700ul) of Tris-saturated phenol, and shake and mix on a DNA mixer for 30 minutes, taking care not to shake left and right. The Eppendorf tube was placed in a centrifuge, and centrifuged at 12,000 rpm for 10 min ...
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The invention discloses a silver carp label release and release effect evaluation method, specifically: dividing the released silver carp into large individual silver carp and small individual silver carp, implanting microsatellite markers in the abdominal cavity of the large individual silver carp, and implanting microsatellite markers in the abdominal cavity of the small individual silver carp. Fix the external tag on the dorsal fin of the released silver carp; sample the fin clipping rays of the released silver carp and record the information of the silver carp; use microsatellite marker technology to identify the genetic information of each silver carp, count the alleles of each released silver carp and construct the sample genetic information Archives; after releasing silver carp, place satellite signal receivers at fixed points in the river to collect satellite signals, determine the activity range of released silver carp, and recapture silver carp in the released area during the fishing period; identify the recaptured individuals and make statistical returns The number of released individuals in silver carp catches. This method can effectively identify the proportion of released silver carp in the released waters, and then evaluate the release effect of silver carp, which has great promotion value.
Description
technical field [0001] The invention belongs to the technical field of proliferation and discharge, and in particular relates to a silver carp marker discharge and a method for evaluating discharge effect. Background technique [0002] With the increase in the range of human activities, the living environment of fish has been greatly affected, and the species and quantity of fish have declined sharply. Proliferation and release are the activities of artificially releasing aquatic species or broodstock to public waters such as oceans, rivers, and lakes. The effect of proliferation and release should be said to be very good. It can be reflected in several aspects. The first aspect is that through proliferation and release, groups of biological resources can be replenished and restored, which increases resources and improves the population structure of organisms. Maintain biological diversity. Some endangered species can increase their numbers by multiplying and releasing the...
Claims
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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2565/125
Inventor 王俊蒋小明
Owner XIAN UNIV OF TECH