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Sealing agent for biological detection, preparation method thereof, coated plate and kit using the coated plate

A technology of biological detection and sealing agent, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of high false positive results of kits, achieve the effects of reducing non-specific adsorption, improving detection accuracy, and reducing false positive results

Active Publication Date: 2021-12-17
北京金诺百泰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to improve the diagnostic specificity and solve the problem of high false positive results of the kit, the application provides a sealing agent for biological detection and its preparation method, a coated plate and a kit using the coated plate

Method used

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  • Sealing agent for biological detection, preparation method thereof, coated plate and kit using the coated plate
  • Sealing agent for biological detection, preparation method thereof, coated plate and kit using the coated plate
  • Sealing agent for biological detection, preparation method thereof, coated plate and kit using the coated plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] It is prepared by uniformly mixing 5.8 mg of water-soluble alcohol organic matter, 0.2 mg of dithiothreitol, 0.1 mg of sodium azide and 993.9 mg of triple distilled water per milliliter of blocking agent for biological detection. Wherein the water-soluble alcoholic organic substance is 4.3 mg of ethylene glycol and 1.5 mg of polyethylene glycol with a relative molecular weight of 400.

Embodiment 2

[0044] The difference from Example 1 is that the water-soluble alcoholic organic substances are 2.9 mg of polyvinyl alcohol and 2.9 mg of polyethylene glycol, and the rest are the same as in Example 1.

Embodiment 3

[0046] The difference from Example 1 is that the water-soluble alcoholic organic substances are 2 mg of ethylene glycol, 1.8 mg of polyvinyl alcohol and 2 mg of polyethylene glycol, and the rest are the same as in Example 1.

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PUM

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Abstract

The application relates to the field of biological detection, and specifically discloses a sealing agent for biological detection, a preparation method thereof, a coated plate and a kit using the coated plate. Each liter of sealing agent is made of the following raw materials: 2.5-9g of water-soluble alcoholic organic matter, 0-1.1g of polyglucosamine, 0-0.7g of gelatin, 0.1-0.4g of antioxidant, 0.07-0.2g of preservative, and the balance It is water; the water-soluble alcoholic organic matter is selected from at least two of ethylene glycol, polyvinyl alcohol and polyethylene glycol. The preparation method of the sealing agent comprises the following steps: uniformly mixing water-soluble alcoholic organic matter, polyglucosamine, gelatin, antioxidant, preservative and water according to the formula quantity. The coated plate is prepared after being sealed with the above sealing agent, and the kit includes the above prepared coated plate. The use of the blocking agent of the present application can effectively reduce the non-specific reaction in the detection process, with few false positive results and high diagnostic specificity.

Description

technical field [0001] The application relates to the field of biological detection, more specifically, it relates to a sealing agent for biological detection, a preparation method thereof, a coated plate and a kit using the coated plate. Background technique [0002] The basis of solid-phase blocking ELISA is the immobilization of antigen or antibody and the enzyme labeling of antigen or antibody. The antigen or antibody bound to the surface of the solid phase carrier still maintains its immunological activity, and the enzyme-labeled antigen or antibody retains its immunological activity. chemical activity while retaining enzymatic activity. [0003] In the preparation of ELISA antibody kits, in general, soluble antigens are adsorbed on solid-phase carriers such as polystyrene, and the antibodies in the serum react specifically with the antigens to form antigen-antibody immune complexes. Since the adsorption between the soluble antigen and the solid-phase carrier is non-sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/54393
Inventor 吴星亮王亚玲金玲文晶亮
Owner 北京金诺百泰生物技术有限公司
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