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Bacillus cereus and use thereof

A technology of Bacillus cereus and Bacillus, applied in the field of microorganisms, can solve the problems of not being able to meet various needs and effectively degrade fucoidan, and achieve the effect of improving the efficiency of screening bacteria

Active Publication Date: 2021-08-20
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the culture medium used by the strains capable of degrading fucoidan does not use fucoidan as the sole carbon source, which will result in the corresponding strains not being able to effectively degrade fucoidan, and the strains screened in the prior art can only degrade fucoidan into Oligosaccharides with two degrees of polymerization cannot meet various needs

Method used

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  • Bacillus cereus and use thereof
  • Bacillus cereus and use thereof
  • Bacillus cereus and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and identification of strains

[0028] (1) Sample collection: The sample is homemade sauerkraut and sour fruit fermented yeast solution in Nanning, Guangxi, sealed in a sterile sealed bag and stored in a -20°C refrigerator;

[0029] (2) Strain activation: after thawing the fermented yeast liquid, spread it on the MRS solid medium respectively, and cultivate it at 37°C for 48 hours;

[0030] (3) Isolation of single colonies: Pick colonies with different shapes on the plate to streak on the MRS solid medium, culture at 37°C for 48 hours, repeat this process more than three times until pure colonies are cultivated;

[0031] (4) Strain screening: inoculate the pure colony in step (3) by streaking on the MRS-H solid medium, and observe the growth of the strain after culturing at 37° C. for 48 h;

[0032] (5) Strain purification and enrichment culture: Select a strain with good growth status in step (4), mark this strain as XY-1, pick a single colony and streak it ...

Embodiment 2

[0037] Degradation of Fucoidan

[0038] (1) Inoculate the Bacillus cereus XY-1 obtained in Example 1 into MRS liquid medium and cultivate it for 48 hours. After the cultivation, centrifuge under the conditions of 10000g and 10min. Take the supernatant and concentrate it to its original volume by rotary evaporation at 40°C 1 / 5 of that, this is the crude enzyme solution;

[0039] (2) Crude enzyme preparation: Prepare saturated ammonium sulfate solution, adjust the pH to be consistent with the crude enzyme solution with ammonia water (the pH of the crude enzyme solution is 4.6), slowly add saturated ammonium sulfate solution dropwise to the crude enzyme solution under ice bath conditions Until obvious precipitation appears in the liquid, let stand overnight at 4°C and then centrifuge. Centrifugation conditions: 10000g, 30min. Same), obtain crude enzyme after freeze-drying;

[0040] (3) Degradation of fucoidan: dissolve 1 g of fucoidan with a molecular weight > 10 kDa in 100 mL ...

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Abstract

The invention discloses bacillus cereus. The bacillus cereus is named as bacillus cereus XY-1 and deposited in Guangdong Microbial Culture Collection Center on December 23, 2020, and has the deposit address of the fifth floor, No.9 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou and the deposit number of GDMCC NO:61383. The obtained bacillus cereus XY-1 through screening uses fucoidan as the unique carbon source, can ensure that all bacteria growing on a culture medium can utilize the fucoidan, and therefore improve screening efficiency of the bacteria. The strain can degrade fucoidan into low-molecular-weight oligosaccharide and can meet various requirements after separation and purification.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus cereus and its application. Background technique [0002] In marine organisms, marine algae have attracted more and more attention as a new source of bioactive substances. Seaweed is divided into three categories: red algae, brown algae and green algae. Brown algae is the second largest group of marine algae and is named for its green-brown phycoxanthin. Fucoidan is a widely used natural product with high added value and high activity. Brown algae have large reserves in the ocean and are renewable. The separation, extraction, structure determination and physiological activity of fucoidan have become the main content of current research. [0003] Fucoidan widely exists in various brown algae. A large number of studies have shown that fucoidan has various health functions, such as anticoagulation, lowering blood sugar, lowering blood lipids, clearing to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/14C12R1/085
CPCC12P19/14
Inventor 高洁陆星宇方芳万楚赵裕民
Owner GUANGXI UNIV
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