A strain of Bacillus cereus and its application

A technology of Bacillus cereus and microbial strains, applied in the field of microorganisms, can solve the problems of not being able to meet various needs and effectively degrade fucoidan, and achieve the effect of improving the efficiency of bacteria screening

Active Publication Date: 2022-07-05
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the culture medium used by the strains capable of degrading fucoidan does not use fucoidan as the sole carbon source, which will result in the corresponding strains not being able to effectively degrade fucoidan, and the strains screened in the prior art can only degrade fucoidan into Oligosaccharides with two degrees of polymerization cannot meet various needs

Method used

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  • A strain of Bacillus cereus and its application
  • A strain of Bacillus cereus and its application
  • A strain of Bacillus cereus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and identification of strains

[0028] (1) Sample collection: The sample is the self-made sauerkraut and sour fruit yeast liquid of Nanning farmhouse in Guangxi, which is sealed in a sterile sealed bag and stored in a -20 ℃ refrigerator;

[0029] (2) Activation of strains: after thawing the yeast liquid, spread it on MRS solid medium, and cultivate at 37°C for 48 hours;

[0030] (3) Isolation of a single colony: Pick the colonies with different shapes on the plate and streak them on the MRS solid medium, and culture at 37°C for 48 hours. This process is repeated more than three times until pure colonies are cultivated;

[0031] (4) Strain screening: The pure colonies in step (3) were streaked on MRS-H solid medium, and the growth of the strains was observed after culturing at 37°C for 48 hours;

[0032] (5) Strain purification and enrichment culture: Select the strain with good growth condition in step (4), mark this strain as XY-1, pick a single colony for s...

Embodiment 2

[0037] Degradation of Fucoidan

[0038] (1) Identification of Example 1 Bacillus cereus XY-1 was inoculated in MRS liquid medium and cultured for 48 hours. After the culture, centrifugation was carried out. The centrifugation conditions were 10000g and 10min. The supernatant was taken and concentrated to 1 / 5 of the original volume by rotary evaporation at 40°C. This was the crude enzyme liquid;

[0039] (2) Preparation of crude enzyme: prepare a saturated ammonium sulfate solution, adjust the pH to the same as the crude enzyme solution with ammonia water (the pH of the crude enzyme solution is 4.6), and slowly add saturated ammonium sulfate solution dropwise to the crude enzyme solution under ice bath conditions Until obvious precipitation appears in the liquid, stand at 4°C overnight and then centrifuge, centrifugation conditions: 10000g, 30min, the precipitate obtained after centrifugation is redissolved in 2-3 times the volume of phosphate buffer (pH and crude enzyme soluti...

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Abstract

The present invention discloses a strain of Bacillus cereus named Bacillus cereus XY-1, which was preserved in the Guangdong Provincial Microbial Culture Collection Center on December 23, 2020, and the preservation address is Building 9, Yard, No. 100, Middle Xianlie Road, Guangzhou The 5th floor, the deposit number is GDMCC NO: 61383. The Bacillus cereus XY-1 obtained by the screening of the present invention uses fucoidan as the only carbon source, which can ensure that the bacteria growing on the medium can utilize the fucoidan, thereby improving the screening efficiency; the strain can degrade the fucoidan into low molecular weight The oligosaccharides can meet various needs after separation and purification.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus cereus and its application. Background technique [0002] Among marine organisms, marine algae have attracted more and more attention as a new source of bioactive substances. Seaweed is divided into three categories: red algae, brown algae and green algae. Brown algae is the second largest group of marine algae and is named for its green-brown phycophytonin. Fucoidan is a widely used, high value-added and highly active natural product. Brown algae have large reserves in the ocean and can be regenerated, making the separation, extraction, structure determination and physiological activity of fucoidan become the main content of current research. [0003] Fucoidan is widely found in various brown algae. A large number of studies have shown that Fucoidan has various health care functions, such as anti-coagulation, lowering blood sugar, lowering blood lipi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/14C12R1/085
CPCC12P19/14
Inventor 高洁陆星宇方芳万楚赵裕民
Owner GUANGXI UNIV
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