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Application and application method of a kind of pcagp7-1 gene in regulating pear dwarfing

An application method and gene technology, applied in the field of genetic engineering, can solve the problems of long breeding time and difficulty in obtaining results.

Active Publication Date: 2022-03-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a method aimed at solving the problem that the existing pear plant dwarfing method has a long conventional breeding time and is difficult to obtain results in a short period of time.

Method used

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  • Application and application method of a kind of pcagp7-1 gene in regulating pear dwarfing
  • Application and application method of a kind of pcagp7-1 gene in regulating pear dwarfing
  • Application and application method of a kind of pcagp7-1 gene in regulating pear dwarfing

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Embodiment 1

[0029] This embodiment provides the experimental results of the overexpression of the PcAGP7-1 gene in the Qiuzi pear plant. Specifically, the method for promoting the dwarfing of the pear plant includes the following steps:

[0030] Monoclonal amplification of S1, PcAGP7-1 gene:

[0031] (1) PCR amplification of the PcAGP7-1 gene:

[0032] Using the upstream primer F and the downstream primer R as the primer pair, use the 40 μL system in the following table 1 to perform monoclonal amplification of the PcAG P7-1 gene to obtain the PcAGP7-1 nucleotide duplex; wherein, the CDS of the PcAGP7-1 gene 序列如序列表SEQ IDNO:1所示,具体为:ATGGCTGCTGCAAAGATAAACCCACTTATCCTGTCAGCTCTCTTTTTGTTCTCAATAATTCTCAGCTTTCCTGCAACAACAATATCAGATAATCCCTGTGCATACCCATGTTATCCTCCACCTACAGGCACCGGGAGCACTTCTACTCCAACAACAACAACTCCGACAACCACAACTCCATCTCCTCCGTCGTCCCAAACCGGGTCATACCCGCCTCCGGCCGTCGTCAACAACCCAAATGGAAATTACCCGTACAACCCTCCTCCGTCTACTGATAACGGTGGTTATGGATATGGTGCTAATCCTCCTCCTGACCCCATCTTACCTTATTTTCCATTCTACTTCAAAAACGGTGCTCGTGGAAAC...

Embodiment 2

[0064] The two-month-old rooted transgenic plants and control plants obtained in Example 1 above were transferred to the substrate for phenotypic observation and physiological data determination, which specifically included the following steps:

[0065] (1) Select 6 two-month-old transgenic Qiuzi pear plants with the same growth and 6 non-transgenic Qiuzi pear plants in the control group.

[0066] (2) Put the sterilized vermiculite into square seedling pots (length and width are 8×8cm, and depth is 10cm), two groups of flowerpots containing plants are put into trays of 30×20cm respectively, and the above-mentioned The Qiuzi pear plant seedlings were moved to flowerpots respectively, and the trays were poured with nutrient solution and cultivated for 40 days.

[0067] (3) Measure each group of plants before and after 40 days of treatment, plant height, internode distance, root length, anatomical structure, chlorophyll content, leaf photosynthetic rate and statistical analysis. ...

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Abstract

The present invention is applicable to the technical field of genetic engineering and provides a PcAGP7-1 The application and application method of genes in regulating the dwarfing of pears, the present invention adopts the form of transgenic plants to PcAGP7-1 Overexpression in pear plants can regulate the growth and development process of pear plants, promote the dwarfing of pear plants, solve the problem of lack of pear dwarfing resources, and thus promote the application of pear plants in dwarfing and dense planting production. The invention can promote the dwarfing of pear plants, greatly shortens the breeding period compared with the traditional hybrid breeding method, can quickly obtain dwarfed plants, is simple and easy to operate, and has good stability.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an application and application method of PcAGP7-1 gene in regulating pear dwarfing. Background technique [0002] Dwarfing of fruit trees is an extremely important agronomic trait, which has the advantages of early fruiting, high yield, and easy management. In recent years, dwarf cultivation has become the development direction of fruit tree cultivation in the world. Therefore, the cultivation and utilization of dwarf fruit tree varieties has important application value. At present, dwarf rootstocks and dwarf varieties of apples have been widely used in production, but the lack of dwarf varieties of pears seriously restricts the application of dwarf and dense planting models in production. [0003] There are two main ways to realize the dwarfing of fruit trees in production: grafting dwarf rootstocks and planting dwarf varieties. As one of the fruits wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/84A01H5/04A01H6/74
CPCC07K14/415C12N15/8205C12N15/8261
Inventor 郑晓东李玉超王彩虹田义轲孙志娟马长青刘晓丽
Owner QINGDAO AGRI UNIV
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