Method for accurately and visually constructing stem cell gel in situ and matched diagnosis and treatment system

A diagnosis and treatment system and stem cell technology, applied in the field of precise visualization of in situ construction of stem cell gels, can solve problems such as difficulty in cell survival and low tissue repair efficiency, and achieve the effect of improving gel forming efficiency and realizing regeneration and repair technology.

Active Publication Date: 2021-10-01
戴江华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulty in blood supply reconstruction and the toxic effects of inflammatory factors and oxygen free radicals on the seed cells, it is difficult for the cells to survive and the efficiency of tissue repair is low. , Cell nutrition is pumped into the original site of the tissue defect, and at the same time, the local inflammatory factors, oxygen free radicals, metabolic wastes, etc.

Method used

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  • Method for accurately and visually constructing stem cell gel in situ and matched diagnosis and treatment system
  • Method for accurately and visually constructing stem cell gel in situ and matched diagnosis and treatment system
  • Method for accurately and visually constructing stem cell gel in situ and matched diagnosis and treatment system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Basic as attached figure 1 to attach Figure 7 As shown, a supporting diagnosis and treatment system for accurately and visually constructing stem cell gel in situ includes a micro-injection pump 1. The micro-injection pump 1 includes a display screen 11, a setting button 12, a foot switch 14, and a drive motor 13. The micro-injection pump 1 is an existing device, and a syringe 15 driven by a drive motor 13 is installed on the microinjection pump 1 .

[0057] In this embodiment, an outer trocar needle 21 is also included. The outer trocar needle 21 includes a needle handle 211. The needle handle 211 is provided with an interface I214 and an interface II215. The needle handle 211 is fixed with a needle body 212 communicating with the interface I214 and the interface II215. .

[0058] In this embodiment, when the outer trocar needle 21 is combined with the existing puncture needle 26, as attached Figure 4 As shown, the outer trocar 21 is detachably connected to the pu...

Embodiment 2

[0082] The difference between the second embodiment and the first embodiment is that the attached Figure 8 And attached Figure 9 As shown, the difference is between S4 and S5.

[0083] S4: Use a set of diagnosis and treatment trocars 2 to puncture into one side of the tissue, take out the puncture needle 26, connect the optical fiber base 24 to the trocar interface II215, and connect the plug 25 to the interface I214. Use another group of diagnosis and treatment trocars 2 to puncture to the inside of the other side of the tissue, take out the puncture needle 26, connect the infusion connector 22 on the outer needle interface II215, and connect the plug 25 to the interface I214.

[0084] S5: Turn on the white light source device 35, and the white light source device 35 irradiates white light through the beam splitter 31, the optical cable 32, the optical fiber base 24 connected to the diagnosis and treatment trocar 2, and the head of the optical cable rod 37, and then the co...

Embodiment 3

[0087] This embodiment provides a gel with strong adhesion, and the preparation method includes the following steps:

[0088] S1: (PRP / GelMA solution) gel solution preparation:

[0089] (1) Configure 0.5% (w / v) photoinitiator LAP solution

[0090] S11: Weigh 0.05g of LAP on an electronic balance and put it into a brown bottle

[0091] S12: Take 10ml of PBS solution and add it into a brown bottle containing 0.05g of photoinitiator for later use

[0092] S13: Heat and dissolve in a water bath at 40-50°C for 15 minutes, shaking several times during the period

[0093] (2) The configuration concentration is 5% (w / v) GelMA solution

[0094] S11: Prepare two clean centrifuge tubes, marked as A and B respectively, and wrap them in tin foil to avoid light

[0095] S12: Weigh 0.25g of GelMA gelatin on an electronic balance and put them into centrifuge tubes A and B

[0096] S13: Use a pipette gun to take the above 2.5ml prepared 0.5% (w / v) photoinitiator solution and 2.5ml PBS sol...

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Abstract

The invention relates to the technical field of tissue and organ regeneration, and discloses a method for accurately and visually constructing stem cell gel in situ and a matched diagnosis and treatment system which comprises a micro-injection pump, a diagnosis and treatment trocar and an injector installed on the micro-injection pump. The diagnosis and treatment trocar comprises an outer trocar and an infusion connector, the infusion connector communicates with the injector, an optical cable is arranged on the diagnosis and treatment trocar, and an optical fiber seat detachably connected with the diagnosis and treatment trocar and an optical cable rod in sliding fit with the outer trocar are arranged on the optical cable; and the optical cable is connected with an optical splitter, the optical splitter is connected with a camera device, a white light source device and a curing light source device, and the camera device is electrically connected with a display. The structure is simple, a PRP / GelMA solution can be conveniently injected into an affected part to form a gel scaffold, and the gel scaffold plays an important role in tissue regeneration, shaping and functional reconstruction.

Description

technical field [0001] The invention relates to the technical field of tissue and organ regeneration, in particular to a method for constructing stem cell gel in situ with precise visualization and a supporting diagnosis and treatment system. Background technique [0002] The regeneration and repair of large tissue defects is extremely difficult, and autologous tissue transplantation will cause bleeding, infection and new damage, and the source is limited. In recent years, engineered tissue transplantation with mesenchymal stem cells (MSC) as the core has become a research hotspot in the treatment of large-volume diseased tissues. However, due to the difficulty in blood supply reconstruction and the toxic effects of inflammatory factors and oxygen free radicals on the seed cells, it is difficult for the cells to survive and the efficiency of tissue repair is low. , Cell nutrition is pumped into the original site of the tissue defect, and at the same time, the local inflamma...

Claims

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Application Information

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IPC IPC(8): A61B17/34A61B90/00A61B1/317A61B1/04A61B1/07A61B90/30A61L27/20A61L27/22A61L27/36A61L27/50A61L27/52A61L27/54
CPCA61B17/3403A61B17/3478A61B17/3472A61B90/361A61B90/37A61B1/317A61B1/04A61B1/07A61B90/30A61L27/222A61L27/20A61L27/3616A61L27/365A61L27/3641A61L27/50A61L27/52A61L27/54A61B2090/306A61L2430/34A61L2430/02A61L2430/24A61L2430/38A61L2300/412C08L5/08
Inventor 戴江华曹钟流杨慧春吴嘉强罗军
Owner 戴江华
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