Combined pharmaceutical composition for enhancing tumor immunity and application thereof

A composition and drug technology, applied in the field of drugs, to achieve the effects of releasing immunosuppressive effects, enhancing infiltration, and increasing solubility

Active Publication Date: 2021-11-02
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few detailed reports on the research on the compatibility of the two in tumor treatment and application.

Method used

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  • Combined pharmaceutical composition for enhancing tumor immunity and application thereof
  • Combined pharmaceutical composition for enhancing tumor immunity and application thereof
  • Combined pharmaceutical composition for enhancing tumor immunity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of co-loaded nanoemulsions of rhein and berberine

[0038] The preferred ratio of rhein and berberine co-loaded nanoemulsion of the present invention is (rhein: berberine=1:1):

[0039] Rhein 0.09 g berberine 0.09 g cinnamon oil 5.0 g Polyoxyethylene hydrogenated castor oil EL35 9.0 g polyethylene glycol 400 6.0 g

[0040] Dilute to the required volume with water for injection.

[0041] Weigh the prescribed amount of cinnamon oil, polyoxyethylene hydrogenated castor oil EL35, polyethylene glycol 400, rhein and berberine, put them in a 50ml beaker, stir at 200r / min at room temperature until the system is in a homogeneous phase, slowly add and inject Form a nanoemulsion with water, which can be diluted to the required volume with water for injection.

[0042] The optimal ratio of rhein and berberine co-loaded nanoemulsion of the present invention is (rhein: berberine=1:100):

[0043] Rhein 0.002 g...

Embodiment 2

[0051] Example 2: Characterization of co-loaded nanoemulsions of rhein and berberine

[0052] The prepared sample was diluted to a certain extent, and its average particle size and Zeta potential were measured with a laser particle size analyzer. After the sample was dropped on the copper grid, it was negatively stained with 2% (w / v) phosphotungstic acid, and the morphology and particle size of the nanoemulsion were observed through a transmission electron microscope (TEM).

[0053] Test results such as figure 1 and figure 2 Shown: after 2% phosphotungstic acid negative staining electron micrograph, it can be seen that rhein and berberine co-load nanoemulsion ( figure 1 ) with a complete surface, a circular shape, uniform particle size distribution, and the particle size is about 200nm. Rhein and berberine co-loaded nanoemulsion ( figure 2 ) particle size distribution, it can be seen that the particle size distribution of the nanoemulsion is uniform, the average particle...

Embodiment 3

[0054] Example 3: Stability study of co-loaded nanoemulsions of rhein and berberine

[0055] The effect of high-speed centrifugation on the stability of rhein and berberine co-loaded nanoemulsions was investigated. After high-speed centrifugation, compared with untreated nanoemulsions, drug precipitation, flocculation and stratification did not occur, and the particle size and PDI did not change. Significant difference ( image 3 A), showing that the stability of rhein and berberine co-loaded nanoemulsions is not affected by high-speed centrifugation.

[0056] The effects of heating and cooling cycles on the stability of rhein and berberine co-loaded nanoemulsions were investigated. After the heat-cold cycle test, the particle size and PDI of the nanoemulsions were not significantly different from those of untreated ( image 3 B), rhein and berberine co-loaded nanoemulsions have good stability at 4°C and 25°C.

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Abstract

The invention discloses a combined pharmaceutical composition for enhancing tumor immunity and an application of the combined pharmaceutical composition. The combined pharmaceutical composition comprises rhein and berberine. The rhein and berberine are combined for application, the antitumor activity is higher than that of a single component, the combined pharmaceutical composition can remarkably enhance the antitumor effect of an immune checkpoint inhibitor, the lifetime of tumor-bearing mice can be prolonged, and a new method and thought are provided for cancer treatment.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a combined pharmaceutical composition for enhancing tumor immunity and its application. Background technique [0002] Malignant tumors are one of the major reasons that threaten human life safety. At present, the main treatment methods include surgery, chemotherapy, radiotherapy, targeted therapy and immunotherapy. Among them, tumor immunotherapy has become a research hotspot in recent years. Immunotherapy such as PD-L1 monoclonal antibody Checkpoint inhibitors have gradually entered clinical trials. However, many patients still have poor response to immune checkpoint inhibitors alone, so a large number of studies are exploring the combined application of immune checkpoint inhibitors with chemotherapy and radiotherapy. Due to the high toxicity and side effects of many chemotherapy and radiotherapy methods, it is of great significance to find drugs and dosing regimens with hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61K31/192A61K45/06A61K9/107A61P35/00
CPCA61K31/192A61K31/4375A61K45/06A61K9/1075A61K47/44A61P35/00A61K2300/00Y02A50/30
Inventor 熊阳赵悦钱柯马莉莎张红艳施经斌许淑君
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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