Fusarium strain ZH-H2 and application of fusarium strain ZH-H2 in degradation of organic matters

A ZH-H2, sickle technology, applied in the fields of application, organic fertilizer, water pollutants, etc., can solve the problem of unsatisfactory degradation effect of high-ring polycyclic aromatic hydrocarbons

Active Publication Date: 2021-11-05
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past, most of the microorganisms that degrade aromatic hydrocarbons were aimed at low-molecular-weight PAHs. There were few dominant strains that could degrade high-ring PAHs, especially those with five or more rings, and the degradation effect on high-ring PAHs was not ideal.

Method used

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  • Fusarium strain ZH-H2 and application of fusarium strain ZH-H2 in degradation of organic matters
  • Fusarium strain ZH-H2 and application of fusarium strain ZH-H2 in degradation of organic matters
  • Fusarium strain ZH-H2 and application of fusarium strain ZH-H2 in degradation of organic matters

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Embodiment 1

[0031] The bacterial strain of the present invention, its bacterial strain preservation name is: ZH-H2, classification name is: Fusariumdlaminii, depository unit: China Microorganism Culture Preservation Management Committee General Microbiology Center, preservation date: June 11, 2014, preservation number: CGMCCNo. 9316. See the prior patent CN104357331A of the inventor team.

Embodiment 2

[0033] After the bacterial strain of the present invention is cultured at 30 DEG C on the potato dextrose agar PDA medium for 4 days, the diameter of the bacterial colony is 3.2 cm; Cotton-like; conidia are sickle-shaped, slightly curved, both ends acuminate, 0-3 septa, most without septa, sporogenous cells are single phialide; chlamydospores are spherical.

Embodiment 3

[0035] The method for obtaining the bacterial strain comprises the following steps: (1) Sampling From the grassland around the Tangshan coal mining area, one part of the 0-10cm surface layer mixed sample is taken according to the plum-blossom sampling method. Complete steps (2)-(4) screening strains within 24 hours after taking. The coal mining area mentioned here refers to the coal mining area that contains factories such as iron and steel plants and coking plants. (2) Enrichment Take 1 g of the mixed sample, use inorganic salt liquid medium to obtain enriched bacterial liquid, and take 1 mL of enriched liquid to inoculate into Gao's No. 1 medium for cultivation. Among them, the inorganic salt medium includes: MgSO4.7H2O0.2g, CaCl2.2H2O0.02g, FeSO4.7H2O0.01g, KH2PO40.4g, Na2HPO40.6g, MnSO4.H2O0.02gNH4NO31.0g, distilled water 1L. Gaoshi No. 1 medium includes: soluble starch 2g, sodium chloride 0.05g, potassium nitrate 0.11g, dipotassium hydrogen phosphate 0.05g, magnesium sul...

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Abstract

The invention discloses a fusarium strain ZH-H2 and an application of the fusarium strain ZH-H2 in degradation of organic matters. The fusarium strain ZH-H2 is used for carrying out combined degradation or independent degradation on imidacloprid and benzopyrene. The invention also comprises a microbial inoculum product for degrading imidacloprid and benzopyrene, and the microbial inoculum product is a fusarium strain ZH-H2 product or a complex microbial inoculum product containing the fusarium strain ZH-H2. The efficient biodegradation effect of the fusarium strain ZH-H2 on imidacloprid is found and confirmed for the first time, and the fusarium strain ZH-H2 has important application value in the fields of agriculture and forestry crop and food safety and water body and soil environmental protection.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a Fusarium strain capable of degrading various harmful organic substances such as imidacloprid and benzopyrene and its application. Background technique [0002] As the amount and frequency of pesticide use continue to increase, a large amount of pesticide residues accumulate in the soil. As of 2020, taking strawberries as an example, fungicides accounted for 69% of the registered pesticides in my country. Insecticides are the most registered pesticide type except fungicides, accounting for 18% of the registered pesticides, of which imidacloprid is The only registered pesticide detected in the risk assessment of pesticide residues in strawberries for four consecutive years. Imidacloprid has soil retention, and after 3 years of use, residues can still be detected in crops that have not been directly applied. The long-term continuous residue accumulation direct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01N25/32C09K17/14A62D3/02A62D101/04A62D101/20
CPCC02F3/34A01N25/32C09K17/14A62D3/02C02F2101/306C02F2101/34C02F2101/38A62D2101/04A62D2101/20
Inventor 杨志新王小敏宁国辉赵欧亚段亚军冯圣东
Owner HEBEI AGRICULTURAL UNIV.
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