Unlock instant, AI-driven research and patent intelligence for your innovation.

Base coupling efficiency detection method and device

A technology of coupling efficiency and detection method, applied in the field of communication, can solve the problems of unstable DMT detection method and inaccurate and complete data analysis results.

Active Publication Date: 2022-06-21
天津擎科生物技术有限公司 +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a base coupling efficiency detection method, device, electronic equipment and storage medium, which are used to solve the defects that the DMT detection method in the prior art is not stable enough, and the data analysis results are not accurate and complete, and realize the high efficiency and stability of DMT detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Base coupling efficiency detection method and device
  • Base coupling efficiency detection method and device
  • Base coupling efficiency detection method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention. , not all examples. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0046] DNA chemical synthesis proceeds from the 3' end to the 5' end, and the specific synthesis steps are as follows:

[0047] Deprotection group, using trichloroacetic acid to remove the protecting group DMT of the nucleotide connected to the CPG to obtain a free 5'-hydroxyl end for the next condensation reaction.

[0048] Activated, the protonated nucleoside 3'-phosphoramidite monomer is mixed with the tetrazol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a base coupling efficiency detection method and device, wherein the method includes: determining the actual value of the color depth of dimethoxytrityl in the process of synthesizing a base with a preset molar mass; The reference value of the color depth of the dimethoxytrityl group in the base process; determine the coupling efficiency of the base according to the ratio between the actual value and the reference value. The base coupling efficiency detection method and device provided by the embodiments of the present invention determine the actual value and reference value of the DMT color depth change in the base synthesis process in real time, and characterize the base coupling efficiency by the ratio between the actual value and the reference value , for a complete and accurate analysis of the data.

Description

technical field [0001] The present invention relates to the technical field of communications, and in particular, to a method and device for detecting base coupling efficiency. Background technique [0002] In the process of deoxyribonucleic acid (DNA) synthesis, the deblocking group uses trichloroacetic acid (TCA) to remove the nucleotides attached to the controlled pore glass (Controlled Pore Glass, CPG) The protecting group of dimethoxytrityl (DMT) to obtain a free 5'-hydroxyl end for subsequent condensation reactions. [0003] DMT detection mainly detects the color depth after the reaction of dimethoxytrityl, converts the color depth into data for further analysis, and calculates the coupling efficiency of bases. [0004] However, the DMT detection system in the prior art adopts serial communication, which can only be backward compatible with various synthesizer devices, and the detection method is not stable enough, and the data analysis results are not accurate and co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/00G16B15/00G06F17/18G01N21/25
CPCG16B20/00G16B15/00G06F17/18G01N21/25
Inventor 马石金苑永军杜军陈园园
Owner 天津擎科生物技术有限公司