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Method for identifying corn straw carbon assimilation key microorganisms in soil

A technology of corn straw carbon and identification method, which is applied in the directions of biochemical equipment and methods, microbial measurement/inspection, bioinformatics, etc., can solve the problems of identifying or answering the succession law of key microbial groups of straw carbon assimilation, and achieves Promote rapid decomposition, soil improvement, rapid soil effect

Pending Publication Date: 2021-11-16
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for identifying key microorganisms of corn straw carbon assimilation in soil, so as to solve the problem that the prior art cannot identify or answer the key microbial groups of straw carbon assimilation and their evolution in straw decomposition. Alternative technical issues

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  • Method for identifying corn straw carbon assimilation key microorganisms in soil
  • Method for identifying corn straw carbon assimilation key microorganisms in soil
  • Method for identifying corn straw carbon assimilation key microorganisms in soil

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Embodiment

[0039] Example: Identification of key microorganisms that hinder corn stalk carbon assimilation in yellow cinnamon soil

[0040] 1. Materials and methods

[0041] 1.1 Collection of soil samples

[0042] Taking the yellow cinnamon soil wheat-corn rotation system as the research object, the experimental site is located in the Modern Agriculture Demonstration Park of Zhaohe Town, Fangcheng County, Henan Province (33°08′N, 112°58′E), which is in the subtropical continental climate zone. The average sunshine hours are 2092 hours, the average annual temperature is 14.4 ℃, the average annual rainfall is about 803.9 mm, and the frost-free period is 220 days. The test started in the 2012 wheat season, and the organic matter of the test soil (0-20cm) before the test was 22.83 g kg -1 , alkaline hydrolysis nitrogen, available phosphorus and available potassium were 191.0, 46.6 and 99.0 mg kg -1 , pH 5.92. Residential Area 49 m 2 , repeated 3 times, arranged in random blocks. Select o...

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Abstract

The invention discloses a method for identifying corn straw carbon assimilation key microorganisms in soil, and aims to solve the technical problems that in the prior art, straw carbon assimilation key microorganism class groups and succession laws of the straw carbon assimilation key microorganism class groups in straw decomposition cannot be identified or answered on the species level. On the basis of a stable isotope probe technology and separated 13C labeled microorganism DNA, each OTU representative sequence is selected for taxonomic analysis, community composition is subjected to statistical analysis at each level, alpha / beta-diversity and co-occurrence network analysis of the corn straw carbon assimilation bacterial community is carried out, so the straw carbon assimilation key microbial group and the community succession law thereof are identified, and then the microbiological mechanism of straw decomposition in target soil is disclosed, so that a targeted efficient corn straw decomposition agent can be further researched and developed to promote rapid decomposition of straw, and the purposes of improving soil, saving fertilizer and improving efficiency are achieved.

Description

technical field [0001] The invention relates to the technical field of microbial identification and recognition, in particular to a method for identifying key microorganisms for corn stalk carbon assimilation in soil. Background technique [0002] Straw returning to the field is an important way to efficiently utilize nutrient resources and an important guarantee for the sustainable development of farmland. my country produces about 900 million tons of straw every year, and its nutrient content is equivalent to 25% of the total amount of fertilizer nutrients in the country (Liu Xiaoyong and Li Shutian, 2017). Straw returning plays an important role in improving soil fertility, improving soil structure, and enriching soil microbial diversity. [0003] Since the second soil survey, the soil area with a pH value of 6.5 to 7.5 in Henan Province has decreased by 1.8%, but the soil with a pH value of less than 6.5 and 5.5 has increased by 2.51% and 1.29%. One of the typical soil...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/30G06K9/62C12Q1/6869
CPCG16B20/30C12Q1/6869G06F18/23G06F18/24155C12Q2531/113C12Q2535/122
Inventor 张倩王宜伦李慧韩燕来谭金芳
Owner HENAN AGRICULTURAL UNIVERSITY
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