Animal protein enzymolysis and vegetable protein fermentation linked feed preparation method
A technology of animal protease and plant protein, which is applied in the field of feed preparation in which animal protein enzymatic hydrolysis and plant protein fermentation are linked, can solve the problems of difficult animal absorption and high content of anti-nutritional factors, improve digestion and utilization, improve bacterial group structure, improve The effect of immunity
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Embodiment 1
[0031] Take a certain amount of fresh chicken liver or frozen chicken liver (frozen chicken liver needs to be thawed), mince it and grind it into a puree, stir it evenly, seal it and boil it for 30 minutes, add 500U / g compound protease after cooling down, keep it at 50°C for 6 hours . After enzymatic hydrolysis, the sample and dry material were mixed at a ratio of 1:2.5 (dry material: crushed corn 65%, soybean meal 10%, rapeseed meal 5%, bran 20%), and then added 1.5×10 6 cfu / g lactic acid bacteria, 1×10 6 cfu / g yeast, 1.5×10 7 cfu / g compound bacillus and 300 U / g compound protease were thoroughly mixed and cultured at 37°C for 4 days to obtain sample 1.
Embodiment 2
[0033] Take a certain amount of fresh duck intestines or frozen duck intestines (frozen duck intestines need to be thawed), grind them into a puree, stir well, seal and boil for 30 minutes, add 400U / g compound protease after cooling down, and keep warm at 50°C for 6 hours . After enzymatic hydrolysis, the sample and dry material were mixed in a ratio of 1:1.5 (dry material: crushed corn 65%, soybean meal 10%, rapeseed meal 5%, bran 20%), and then added 1×10 6 cfu / g lactic acid bacteria, 1.5×10 6 cfu / g yeast, 1.5×10 7 cfu / g compound bacillus and 200 U / g compound protease were thoroughly mixed and cultured at 37°C for 4 days to obtain sample 2.
Embodiment 3
[0035] Take a certain amount of frozen chicken waste (chicken liver, chicken intestines), thaw it, mince it and grind it into a puree, stir it evenly, seal it and boil it for 30 minutes, add 450U / g compound protease after cooling down, and keep it at 50°C for 6 hours for enzymatic hydrolysis. After enzymatic hydrolysis, the sample and dry material were mixed in a ratio of 1:2 (dry material: crushed corn 65%, soybean meal 10%, rapeseed meal 5%, bran 20%), and then added 1×10 6 cfu / g lactic acid bacteria, 1×10 6 cfu / g yeast, 1×10 7 cfu / g compound bacillus and 250 U / g compound protease were thoroughly mixed and cultured at 37°C for 4 days to obtain sample 3.
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