Holdemanella sp. bacterium and use thereof

A technology of bacteria, uses, applied in the field of HOLDEMANELLA SP. bacteria and uses, which can solve the problems of limited effectiveness, single treatment target, and no solution to obesity and the complexity of its complications

Pending Publication Date: 2021-12-14
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, their effectiveness is limited because they are based on a single therapeutic goal without addressing the complexity of the mechanisms leading to obesity and its complications

Method used

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  • Holdemanella sp. bacterium and use thereof
  • Holdemanella sp. bacterium and use thereof
  • Holdemanella sp. bacterium and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1. Isolation and identification of Holdemanella biformis bacteria.

[0094] Different gut bacteria were isolated from the feces of healthy volunteers. 1.25 g of feces were used and diluted in 10 mM phosphate buffered saline containing 0.05% cysteine ​​(1:10 dilution) with a NaCl concentration of 130 mM (PBS) and processed on a Lab-Blender Stomacher 400 (Seward Medical, London, 35UK) for homogenization. Said dilution was inoculated in 37.5 ml of intestinal bacteria medium (IBM), the composition of which was based on the medium recommended in previous publications (Gibson, G.R., et al., Appl. Environ. Microbiol., 54(1 1) :2750-5, 1988; Lesmes, U et al., J.Agric.FoodChem., 56:5415-5421, 2008), some modifications devised by the inventors:

[0095] - main ingredient : Distilled water (1600ml), peptone water (4g), NaHCO 3 (4 grams), CaCl 2 (0.02g), Pectin (4g), Xylan (4g), Wheat Bran Extract (4g), Arabinogalactan (2g), Arabic Gum (2g), Starch (10g), Cheese Egg ...

Embodiment 2

[0107] Example 2. Selection of H. biformis based on its ability to regulate inflammation in vitro.

[0108] An in vitro assay was performed to comparatively assess the immunomodulatory properties of a collection of bacterial isolates, allowing selection of bacteria capable of inducing the greatest anti-inflammatory response in classical monocytes and, therefore, potentially therapeutic for the treatment of obesity-associated inflammation and alterations in glucose metabolism interest. To this end, cell suspensions of different bacteria were used as stimulators of the number of peripheral blood mononuclear cells (PBMC) and classical monocytes as well as the levels of the anti-inflammatory cytokine IL-4 relative to the pro-inflammatory cytokine IFNγ by flow cytometry technical measurement.

[0109] -Cultivation and stimulation of PBMC

[0110] From whole blood of healthy volunteers, peripheral blood mononuclear cells (PBMC) were isolated using a Ficoll gradient (Ficoll Paque...

Embodiment 3

[0116] Example 3. Characterization of the role of H.biformis in regulating energy and glucose metabolism in an obese animal model

[0117] Development and sampling of animal models of obesity

[0118] Adult male C57BL / 6 mice (6-8 weeks, Charles River, Les Oncins, France) were maintained and fed under controlled temperature (23°C), relative humidity (40-50%) and a 12-hour light / dark cycle A high-calorie diet (HFHSD; D12451, Research Diet, Brogaarden, Denmark) rich in fat (45% Kcal) and sucrose (17% Kcal) or a control diet with a standard fat content and no sucrose (CD, 10% from fat % Kcal; D12450K, study diet, Brogaarden, Denmark) for 14 weeks. Every day, mice fed a HFHSD diet received subjects (1×10 8 colony forming unit [CFU]) oral dose. Vehicle or placebo (10% skim milk) was administered to obese phenotype (HFHSD) control group and lean phenotype (CD) control group (n=10 mice per group) in the same manner. After 14 weeks, mice were sacrificed by cervical dislocation, a...

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Abstract

The present invention relates to the strain Holdemanella biformis, the strain H. biformis CECT 9752, its cellular components, metabolites and secreted molecules, and to compositions comprising the aforementioned products, in addition to the use of said strain, but also of the genus Holdemanella, and to the species H. biformis for preventing alterations in energy homeostasis and glucose metabolism, including, inter alia, glucose intolerance, insulin resistance, metabolic syndrome and diabetes.

Description

technical field [0001] The present invention relates to the Holdemanella genus, the Holdemanella biformis genus and the H. biformis CECT9752 strain, their cellular components, metabolites and secreted molecules, as well as compositions comprising the above-mentioned products, and the use of said strains for the prevention and / or treatment of energy homeostasis and glucose Metabolic Altered Uses. The present invention belongs to the field of therapeutic activity of pharmaceutical compositions or preparations, and also to the field of foods. Background technique [0002] Obesity is currently a major global health challenge due to its high prevalence and comorbidities associated with altered energy homeostasis. These comorbidities include, for example, dyslipidemia, metabolic syndrome, diabetes, cardiovascular disease, hepatic steatosis or fatty liver, hypertension, retinopathy, and changes in eating behavior. These pathologies, especially those involving altered glucose meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P3/04A61P3/08A61P3/10C12R1/01
CPCC12N1/205C12R2001/01A23L33/135A61K2035/115C12N1/20A61K35/74A61P3/04A61P3/10
Inventor Y·桑兹·赫兰兹I·洛佩兹·阿尔梅拉E·M·戈麦斯·德尔·普贾·维拉努瓦A·贝尼特斯-佩兹M·罗曼尼·佩雷斯
Owner CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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