A kind of high-efficiency breeding method of Lanzhou lily seedlings
A seedling and lily technology, applied in the field of lily bulb breeding, can solve the problems affecting the quality and yield of Lanzhou lily, restricting the development of Lanzhou lily industry, and the large number of seeds, so as to achieve high heterogeneity, rapid promotion, and improvement Yield and Quality Effects
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Embodiment 1
[0027] A method for efficient breeding of Lanzhou lily seedlings, comprising the following steps:
[0028] 1) seed seed collection: collect the Lanzhou lily capsule after the flower, remove the pericarp to obtain the seed seed; the collected Lanzhou lily capsule is the more than 50 days after the flower capsule;
[0029] 2) sterilization of seed seeds: after washing the seed seeds containing embryos obtained in step 1), soak them with ethanol solution and sodium hypochlorite solution respectively, then wash with sterile water, and dry the water with sterile filter paper, that is, to obtain a degerming solution. Bacteria seed; first rinse the seed with clean water for 9-11min, then soak it in ethanol solution with a concentration of 70% by volume for 40-50s, and then soak it in a sodium hypochlorite solution with a mass volume ratio of 2% for 10-20min, and finally Washed with sterile water for 3 times, and dried with sterile filter paper, the sterile seeds can be obtained;
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Embodiment 2
[0037] like Figure 1 to Figure 5 As shown, it is a process diagram of taking the Lanzhou lily seed as the explant through debacterial treatment, embryo germination, proliferation culture, strong seedling cultivation and small bulb expansion culture in the present invention.
[0038] 1. Debacterial treatment and germination induction of Lanzhou lily seeds:
[0039] Sterilize the seeds: first rinse with running water for 20 min, soak in 70% (v / v) ethanol for 1 min, then soak in 2% (v / v) NaClO solution for 20 min, and finally wash with sterile water 3 times. Get sterile seeds. The sterile seeds were equally divided into 3 parts, with concentrations of 0 and 250 mg·L, respectively. -1 , 500 mg·L -1 (w / v) gibberellin (GA 3 ) solution was soaked for 24 h, inoculated on 1 / 2 MS medium for dark culture (23-25 °C), and each treatment was repeated three times. The germination rate was counted after 13 days. Table 1 shows GA 3 Effects on the germination rate of Lanzhou lily seed...
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