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Optimized seed potato breeding method

A potato, wild seed technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of complex steps, low germination rate, poor taste, etc. Effect

Inactive Publication Date: 2021-12-31
内蒙古中加农业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, potatoes are used as staple food in many countries and regions, but the planting rate of potatoes is not high at present. Planting potatoes. This will lead to a vicious circle in the development of the potato industry
[0004] At the same time, due to the unique climatic conditions in our country, it takes 4 to 5 years for seed potato breeding to increase the quality and speed of potato breeding. problem, it needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] An optimized seed potato breeding method, the operation steps are as follows:

[0037] Step 1: Take the tissues of wild potato species and cultivated varieties with a seedling age of 28 to 35 days, and cultivate the leaves of virus-free test tube seedlings as materials. Before operation, cut the leaves into thin slices with a blade and place them in the pretreatment solution Carry out the treatment, then put the leaves into the enzymatic hydrolysis solution, and treat them for 20-28 hours under the condition of total darkness at 25°C-30°C, the purification of protoplasts adopts the method of combining floating and sedimentation;

[0038] Step 2: put together the wild species naked cells without cell walls obtained in step 1 and the protoplasts of cultivated species, first use active agents and fusion agents to treat them, and then apply voltage to form electric pulses to make the two Or cell fusion;

[0039] The third step: cultivate the plant tissue culture medium, ob...

Embodiment 2

[0043] An optimized seed potato breeding method, the operation steps are as follows:

[0044] Step 1: Take the tissues of wild potato species and cultivated varieties with a seedling age of 25 to 35 days, and cultivate the leaves of virus-free test tube seedlings as materials. Before operation, cut the leaves into thin slices with a blade and place them in the pretreatment solution Carry out the treatment, then put the leaves into the enzymatic hydrolysis solution, and treat them for 16 to 28 hours under the condition of total darkness at 20°C to 28°C, and the purification of protoplasts adopts the method of combining floating and sedimentation;

[0045] Step 2: put together the wild species naked cells without cell walls obtained in step 1 and the protoplasts of cultivated species, first use active agents and fusion agents to treat them, and then apply voltage to form electric pulses to make the two Or cell fusion;

[0046] The third step: cultivate the plant tissue culture ...

Embodiment 3

[0050] An optimized seed potato breeding method, the operation steps are as follows:

[0051] Step 1: Take the tissues of 35-day-old potato wild species and cultivated varieties, and cultivate the leaves of virus-free test-tube seedlings as materials. Before operation, cut the leaves into thin slices with a blade, and place them in the pre-treatment solution for treatment , and then put the leaves into the enzymatic hydrolysis solution, and treat them for 22 hours at 28°C in total darkness, and the purification of protoplasts adopts the method of combining floating and sedimentation;

[0052] Step 2: put together the wild species naked cells without cell walls obtained in step 1 and the protoplasts of cultivated species, first use active agents and fusion agents to treat them, and then apply voltage to form electric pulses to make the two Or cell fusion;

[0053] The third step: cultivate the plant tissue culture medium, obtain the hybrid plants after screening, adopt the met...

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Abstract

The invention belongs to the technical field of potato breeding, and discloses an optimized seed potato breeding method. The optimized seed potato breeding method comprises the following operation steps that 1, tissues of potato wild species and cultivated species at the seedling age of 25-40 days are taken, leaves of virus-free test-tube plantlets are cultured as materials, the leaves are cut into thin pieces by using a blade before operation, the leaves are placed in a pretreatment solution to be treated, then the leaves are placed in an enzymatic hydrolysate, and the leaves are treated for 16-28 hours under the full-dark condition at the temperature of 20-30 DEG C. According to the optimized seed potato breeding method, wild plants and culture plant cases are combined, so that the hybrid plants which are high in growth and breeding speed of the wild plants and high in quality of the culture plants can be obtained, and the respective defects of the wild plants and the culture plants can be overcome; and meanwhile, by rooting culture and culture of the test-tube plantlets, growth and reproduction of the hybrid plants can be promoted in biological and physical modes correspondingly, so that the hybrid plants which are high in quality, easy to plant in a large area and fast in fruiting are obtained.

Description

technical field [0001] The invention belongs to the technical field of potato breeding, in particular to an optimized seed potato breeding method. Background technique [0002] Potatoes are asexually propagated crops, and tubers are usually used as seeds. Many diseases are easily passed on from generation to generation and expand damage through tubers. Sustainable development and quality improvement of potato industry. [0003] At present, potatoes are used as staple food in many countries and regions, but the planting rate of potatoes is not high at present. Planting potatoes will lead to a vicious circle in the development of the potato industry. [0004] At the same time, due to the unique climatic conditions in our country, it takes 4 to 5 years for seed potato breeding to increase the quality and speed of potato breeding. problem, so it needs to be improved. Contents of the invention [0005] The object of the present invention is to address the above problems, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/06A01H1/02A01H1/00
CPCA01H4/008A01H1/02A01H1/06A01H1/12A01H1/121
Inventor 刘广晶吕文霞李燕于冬梅田艳花候健花孙翠翠包美丽秦昊
Owner 内蒙古中加农业生物科技有限公司
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