Protein-free porcine pseudorabies live vaccine heat-resistant protective agent, and preparation method and application thereof

A technology of porcine pseudorabies and heat-resistant protective agent, which is applied in the direction of biochemical equipment and methods, vaccines, antiviral agents, etc., can solve the problems of reduced immune effect, loss of poison price, poor heat resistance, etc., to achieve improved stability, Low virus loss rate, good anti-aging effect

Active Publication Date: 2022-02-11
山东滨州沃华生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional vaccine freeze-dried products generally use 5% dextran and 10% milk powder as protective agents, which have poor heat resistance, and the storage period at 2-8°C is only 3-6 months, and most of them need to be stored below -15°C
Under the conditions of routine transportation and repeated freezing and thawing, the vaccine has a serious loss of poison value, which greatly reduces its immune effect

Method used

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  • Protein-free porcine pseudorabies live vaccine heat-resistant protective agent, and preparation method and application thereof
  • Protein-free porcine pseudorabies live vaccine heat-resistant protective agent, and preparation method and application thereof
  • Protein-free porcine pseudorabies live vaccine heat-resistant protective agent, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of protein-free porcine pseudorabies live vaccine heat-resistant protective agent

[0023] (1) Dissolve chitosan 0.5%, polyvinylpyrrolidone (PVPK30) 4%, dipotassium hydrogen phosphate 0.2%, and potassium dihydrogen phosphate 0.02% in water for injection, and sterilize at 116°C for 30 minutes to obtain liquid A;

[0024] (2) Dissolve 1% hydroxyethyl starch, 2% calcium lactate, 2.5% dextran, 0.5% arginine, 1% D-sorbitol, and 1% trehalose in water for injection, and use 0.22μm sterile filtration filter sterilization to obtain liquid B;

[0025] (3) Mix equal volumes of liquid A and liquid B to obtain the heat-resistant protective agent.

Embodiment 2

[0026] Embodiment 2: Preparation of protein-free porcine pseudorabies live vaccine heat-resistant protective agent

[0027] (1) Dissolve 1% chitosan, 4% polyvinylpyrrolidone (PVPK30), 0.2% dipotassium hydrogen phosphate, and 0.02% potassium dihydrogen phosphate in water for injection, and sterilize at 116°C for 30 minutes to obtain liquid A;

[0028] (2) Dissolve 1.5% hydroxyethyl starch, 2% calcium lactate, 3% dextran, 0.5% arginine, 1.5% D-sorbitol, and 1.5% trehalose in water for injection, and use 0.22μm sterile filtration filter sterilization to obtain liquid B;

[0029] (3) Mix equal volumes of liquid A and liquid B to obtain the heat-resistant protective agent.

Embodiment 3

[0030] Embodiment 3: Preparation of protein-free porcine pseudorabies live vaccine heat-resistant protective agent

[0031] (1) Dissolve 2% chitosan, 5% polyvinylpyrrolidone (PVPK30), 0.5% dipotassium hydrogen phosphate, and 0.03% potassium dihydrogen phosphate in water for injection, and sterilize at 116°C for 30 minutes to obtain liquid A;

[0032] (2) Dissolve 2% hydroxyethyl starch, 2% calcium lactate, 3.5% dextran, 1% arginine, 2% D-sorbitol, and 2% trehalose in water for injection, and use 0.22μm sterile filtration filter sterilization to obtain liquid B;

[0033] (3) Mix equal volumes of liquid A and liquid B to obtain the heat-resistant protective agent.

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Abstract

The invention relates to the field of veterinary biological product manufacturing, and particularly discloses a protein-free porcine pseudorabies live vaccine heat-resistant protective agent, and a preparation method and application thereof. The porcine pseudorabies live vaccine heat-resistant protective agent is characterized by being composed of a solution A and a solution B which are equal in volume, and the solution A is prepared from chitosan, polyvinylpyrrolidone, dipotassium phosphate, monopotassium phosphate and water; and the solution B is prepared from hydroxyethyl starch, calcium lactate, dextran, arginine, D-sorbitol, trehalose and water. By using the heat-resistant protective agent disclosed by the invention, the storage and transportation requirements of a porcine pseudorabies live vaccine product are reduced, the problem that the porcine pseudorabies live vaccine cannot be stored and transported for a long time at 2-8 DEG C is solved, the stress problem of commercially available heat-resistant protective agent vaccines is solved, and the stability of the product in storage and use is improved.

Description

technical field [0001] The invention relates to the field of veterinary biological product manufacturing, in particular to a heat-resistant protective agent for a protein-free porcine pseudorabies live vaccine and a preparation method and application thereof. Background technique [0002] Porcine pseudorabies is caused by porcine pseudorabies virus, a highly contagious infectious disease of various domestic animals with fever, itching (except pigs), reproductive disorders, and encephalomyelitis as the main symptoms. It is a useful model virus for studying herpes virus biology. The taxonomy of porcine pseudorabies virus is named porcine herpesvirus type I, which belongs to the genus varicellaherpesvirus of the subfamily Herpesviridae. All herpesviruses have a double-stranded DNA genome, virion particles of similar size (200-250nm), similar structure (capsid, envelope, and envelope), and have a latent period in the life cycle. PRVs are also used as "live" tracers of nerve ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/32A61K47/02A61K47/12A61K47/18A61K47/26A61K9/19A61K39/245A61P31/22
CPCA61K47/36A61K47/32A61K47/02A61K47/12A61K47/183A61K47/26A61K9/19A61K39/12A61P31/22A61K2039/552A61K2039/5254C12N2710/16734
Inventor 耿兴良杨灵芝朱杰翟庆贺杨平李香云
Owner 山东滨州沃华生物工程有限公司
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