Sphingobacterium Canadae SC202103 strain for degrading alpha-solanine and application of spingobacterium Canadae SC202103 strain

A technology of sphingosine bacteria and strains, which is applied in the field of microorganisms to achieve the effects of improving adaptability, simple cultivation and strong degradation ability

Active Publication Date: 2022-03-18
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still few reports on other functions of the bacteria at home and abroad.

Method used

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  • Sphingobacterium Canadae SC202103 strain for degrading alpha-solanine and application of spingobacterium Canadae SC202103 strain
  • Sphingobacterium Canadae SC202103 strain for degrading alpha-solanine and application of spingobacterium Canadae SC202103 strain
  • Sphingobacterium Canadae SC202103 strain for degrading alpha-solanine and application of spingobacterium Canadae SC202103 strain

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Embodiment 1

[0028] A strain of Sphingobacter canadenii SC202103 that degrades α-solanine of the present invention, the strain is Sphingobacter canadenica, and the preserved name is Sphingobacter canadenica Glutamicibacter halophytocola; it is preserved in China Microbiological Culture Preservation Management Committee General Microorganisms Center, the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; date of deposit: May 31, 2021; deposit number: CGMCC No.22640.

[0029] The 16S rDNA gene sequence of the α-solanine-degrading Sphingobacter canadenii SC202103 strain is the nucleotide sequence shown in SEQ ID No.1.

[0030] The strain was purified and cultivated by LB plate streaking. It was found that the colony of strain S1 was round, with neat edges, orange yellow, smooth and moist surface, slightly convex in the center, and flat around. Gram-stained red, Gram-negative bacteria, the cells are short rod-shaped.

[0031] The bacterial agent of Sphingobacter ...

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Abstract

The invention discloses a sphingobacterium canadense strain for degrading alpha-solanine and application of the sphingobacterium canadense strain, the sphingobacterium canadense strain for degrading the alpha-solanine is SC202103, the preservation name of the sphingobacterium canadense strain is SC202103, the preservation number of the sphingobacterium canadense strain for degrading the alpha-solanine is Sphingobacterium canadense strain for degrading the alpha-solanine, and the preservation number of the sphingobacterium canadense strain for degrading the alpha-solanine is Sphingobacterium canadense strain for degrading the alpha-solanine is Sphingobacterium canadense strain for degrading the alpha-solanine. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The strain disclosed by the invention is a bacterium capable of being used for degrading alpha-solanine, has the characteristics of strong degradation capability on alpha-solanine and environmental safety, and can be used for biodegradation of alpha-solanine and food safety.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Sphingobacter canadenii SC202103 strain degrading α-solanine, a preparation method and application thereof. Background technique [0002] Solanine, also known as solanine, solanine, potato toxin, etc., is a secondary metabolite glycoalkaloids (Steroidal Glycoalkaloids, SGAs) mainly distributed in Solanaceae plants, such as potato, tomato and eggplant roots, stems, leaf fruits, which are more abundant in potatoes (Friedman and McDonald, 1997; Zhao Xuesong et al., 2007). α-solanine in potato has insecticidal activity (Tingey, 1984; Jonasson and Olsson, 1994; Zheng et al., 2020), and has high toxicity to humans and animals. Ingestion can cause nausea, vomiting, diarrhea, loss of consciousness, Symptoms such as paralysis and shock can cause death if not rescued in time, as well as strong teratogenicity and reproductive toxicity (Ji Yubin, 2009; Tan Jijun, 2018). [0003] A...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
CPCC12N1/20A62D3/02A62D2101/28
Inventor 陈斌王文倩肖关丽杜广祖叶举辉杨宝云薛锐张栩孙淦琳付玉飞
Owner YUNNAN AGRICULTURAL UNIVERSITY
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