Serratia capable of enriching phosphorus, degrading organic phosphorus and inhibiting plant pathogenic fungi

Abstract

The invention discloses serratia capable of enriching phosphorus, degrading organic phosphorus and inhibiting plant pathogenic fungi, and belongs to the technical field of microorganisms. The strain, namely serratia sp. MEW06, is preserved in China Center for Type Culture Collection, with preservation number of CCTCC NO. M2015779. The serratia disclosed by the invention, which has a short-time and efficient phosphorus enriching capacity, can be used for preparing a bio-fertilizer, and the serratia, which also has an inhibitory effect on the pathogenic fungi of crops, can be applied to microbial control; meanwhile, the strain, which has a good effect on degrading organic phosphorus pesticides, namely glyphosate and dimethoate, is capable of effectively eliminating pollution of the organic phosphorus pesticides; and the serratia disclosed by the invention has a broad application scope.

Description

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to Serratia MEW06 and the application of the strain in phosphorus enrichment, organic phosphorus degradation and inhibition of plant pathogenic fungi. Background technique

[0002] Organophosphorus pesticides are a major category of pesticides today, accounting for about 80% of pesticide products. Because of their economical, efficient, and rapid control of agricultural diseases, insect pests, and weeds, they have been mass-produced and widely used at home and abroad. However, due to the expanded production of organophosphorus pesticide chemical plants, the amount of wastewater discharged during the production process is large, the COD content is extremely high, the pollutant components are complex, the toxicity is high, and it is difficult to biodegrade, causing great harm to the environment. Moreover, after the pesticide is applied, it will spread from the appli...

Examples

Embodiment 1

[0026]Water samples from Wuhan Shahu Lake and its surrounding areas were collected and added to LB medium, and enriched at 28°C and 150r / min for 24h. The above bacterial suspension was inoculated into the fermentation medium containing glyphosate 200mg / L according to the inoculum amount of 5%-10%, and after 28°C 150r / min enrichment culture for 3 days, it was transferred to the same medium, under the same conditions Continue to cultivate, and gradually increase the glyphosate content to 1000mg / L during the passage and acclimation process; streak the 5-passage bacterial solution on LB solid medium, culture at 28°C for 24-48h, according to the formation of colonies on the medium , pick well-growing colonies and inoculate them in LB culture solution containing 50mg / L glyphosate (diluted 5 times), culture at 150r / min at 28°C for 72h, and measure by the acetylcholinease inhibition rate method (GB / T5009.199-2003) The content of organophosphorus pesticides was calculated, and the degr...

Embodiment example 2

[0029] After the purified Serratia sp.MEW06 strain was activated overnight, adjust the OD of the bacterial solution 600 =1.0, according to the inoculum size of 4%, it is transferred to culture in the fermentation broth containing a certain concentration of glyphosate or dimethoate, and the degradation rate is measured by sampling at 1d, 3d, and 5d. The formulation of the fermentation medium is as follows: peptone 2g / L, yeast extract 1g / L, NaCl 2g / L, pH7.0-7.2. The results showed that Serratia sp.MEW06 had an excellent effect on the degradation of dimethoate. When treated with low concentration of dimethoate (50mg / L), the concentration of dimethoate was almost undetectable in the supernatant after 5 days of culture, and the degradation rate almost reached 100%. After treating high-concentration dimethoate (200mg / L) and culturing for 7 days, the degradation rate was determined to reach 90.12%. The ability of MEW06 to treat glyphosate was weaker than that of dimethoate, but at t...

Embodiment example 3

[0031] Get the single colony of Serratia in Example 1 and inoculate it into LB medium for overnight activation. The formula of LB medium is as follows: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH7.0-7.2, press 2% of the inoculum was inoculated in PAM medium with a phosphorus content (calculated as P) of 10 mg / L and 20 mg / L respectively, and samples were taken at 0, 12, 24, 48, and 72 hours to determine the phosphorus accumulation rate. The PAM medium formula For: CH 3 COONa 5g / L, NH 4 Cl2g / L, KH 2 PO 4 0.022~0.0878g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCl 2 0.2g / L, 1000mL deionized water, pH7.0~7.2. The results are shown in Table 1. When the phosphorus content in the solution was 10 mg / L, after 24 hours of enrichment, the inorganic phosphorus content was basically not detected in the solution, the phosphorus enrichment rate reached 99.94%, and the phosphorus content in the solution was 20 mg / L. After 24 hours of enrichment, the phosphorus enrichment rate reached 63.79%. T...

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to Serratia MEW06 and the application of the strain in phosphorus enrichment, organic phosphorus degradation and inhibition of plant pathogenic fungi. Background technique

[0002] Organophosphorus pesticides are a major category of pesticides today, accounting for about 80% of pesticide products. Because of their economical, efficient, and rapid control of agricultural diseases, insect pests, and weeds, they have been mass-produced and widely used at home and abroad. However, due to the expanded production of organophosphorus pesticide chemical plants, the amount of wastewater discharged during the production process is large, the COD content is extremely high, the pollutant components are complex, the toxicity is high, and it is difficult to biodegrade, causing great harm to the environment. Moreover, after the pesticide is applied, it will spread from the appli...

Examples

Embodiment 1

[0026]Water samples from Wuhan Shahu Lake and its surrounding areas were collected and added to LB medium, and enriched at 28°C and 150r / min for 24h. The above bacterial suspension was inoculated into the fermentation medium containing glyphosate 200mg / L according to the inoculum amount of 5%-10%, and after 28°C 150r / min enrichment culture for 3 days, it was transferred to the same medium, under the same conditions Continue to cultivate, and gradually increase the glyphosate content to 1000mg / L during the passage and acclimation process; streak the 5-passage bacterial solution on LB solid medium, culture at 28°C for 24-48h, according to the formation of colonies on the medium , pick well-growing colonies and inoculate them in LB culture solution containing 50mg / L glyphosate (diluted 5 times), culture at 150r / min at 28°C for 72h, and measure by the acetylcholinease inhibition rate method (GB / T5009.199-2003) The content of organophosphorus pesticides was calculated, and the degr...

Embodiment example 2

[0029] After the purified Serratia sp.MEW06 strain was activated overnight, adjust the OD of the bacterial solution 600 =1.0, according to the inoculum size of 4%, it is transferred to culture in the fermentation broth containing a certain concentration of glyphosate or dimethoate, and the degradation rate is measured by sampling at 1d, 3d, and 5d. The formulation of the fermentation medium is as follows: peptone 2g / L, yeast extract 1g / L, NaCl 2g / L, pH7.0-7.2. The results showed that Serratia sp.MEW06 had an excellent effect on the degradation of dimethoate. When treated with low concentration of dimethoate (50mg / L), the concentration of dimethoate was almost undetectable in the supernatant after 5 days of culture, and the degradation rate almost reached 100%. After treating high-concentration dimethoate (200mg / L) and culturing for 7 days, the degradation rate was determined to reach 90.12%. The ability of MEW06 to treat glyphosate was weaker than that of dimethoate, but at t...

Embodiment example 3

[0031] Get the single colony of Serratia in Example 1 and inoculate it into LB medium for overnight activation. The formula of LB medium is as follows: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH7.0-7.2, press 2% of the inoculum was inoculated in PAM medium with a phosphorus content (calculated as P) of 10 mg / L and 20 mg / L respectively, and samples were taken at 0, 12, 24, 48, and 72 hours to determine the phosphorus accumulation rate. The PAM medium formula For: CH 3 COONa 5g / L, NH 4 Cl2g / L, KH 2 PO 4 0.022~0.0878g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCl 2 0.2g / L, 1000mL deionized water, pH7.0~7.2. The results are shown in Table 1. When the phosphorus content in the solution was 10 mg / L, after 24 hours of enrichment, the inorganic phosphorus content was basically not detected in the solution, the phosphorus enrichment rate reached 99.94%, and the phosphorus content in the solution was 20 mg / L. After 24 hours of enrichment, the phosphorus enrichment rate reached 63.79%. T...

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