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Mycolicibacteriumneworleansense WCJ and application thereof in degradation of organic pollutants

A technology for organic pollutants and mycobacteria, applied in water pollutants, microorganism-based methods, bacteria, etc., can solve the problems of high-efficiency degradation of mycobacteria not found, and achieve the effect of high-efficiency degradation ability and broad application prospects.

Pending Publication Date: 2021-12-17
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, it is necessary to study the efficient degradation of n-hexane in the environment for human health. Through literature search, it was found that mycobacteria use phenanthrene as the sole carbon source to achieve high-efficiency degradation of phenanthrene, but no reports about mycobacteria using n-hexane as the only Carbon source to achieve high-efficiency degradation reports

Method used

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  • Mycolicibacteriumneworleansense WCJ and application thereof in degradation of organic pollutants
  • Mycolicibacteriumneworleansense WCJ and application thereof in degradation of organic pollutants
  • Mycolicibacteriumneworleansense WCJ and application thereof in degradation of organic pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation, purification and identification of strain WCJ.

[0032] 1. Isolation and purification of strain WCJ.

[0033] Strain WCJ is a Gram-positive bacterium domesticated and isolated from activated sludge. The specific steps are as follows:

[0034]Add 50mL of inorganic salt culture solution to a 300mL shake flask, add 10mL of activated sludge collected from a sewage treatment plant and n-hexane with a final concentration of 30mg / L, and carry out enrichment culture at 30°C until the concentration of n-hexane is the initial addition When the concentration is 50%, take out 5mL of the enrichment solution and put it into 50mL of fresh inorganic salt culture solution, add the same amount of n-hexane (to make the concentration 30mg / L), repeat the above enrichment process 5 times, and then add the enrichment solution for the last time. Dilute the collected solution 1500 times with sterile water, then streak LB solid medium, culture at 30°C, select a single colo...

Embodiment 2

[0049] Embodiment 2, the acquisition of mycobacterium new orleans WCJ resting cell

[0050] 1. Incline cultivation:

[0051] Inoculate Mycobacterium New Orleans WCJ into LB liquid medium, culture at 30°C, 160rpm for 24-36 hours, then line the activated bacteria on a solid LB plate in a 30°C incubator, take a single colony and continue to line the plate In order to detect the purity of the bacteria, the LB test tubes were stored on the inclined plane (4°C) routinely.

[0052] 2. Expand training

[0053] Inoculate the slanted bacteria in step 1 into LB liquid medium, culture at 30°C and 160rpm for 24-36 hours, obtain the expanded culture medium, centrifuge, collect the wet bacteria, wash with inorganic salt culture medium, and obtain Mycobacterium new orleans WCJ resting cells.

Embodiment 3

[0054] Example 3: Detection of the degradation performance of Mycobacterium new orleans WCJ on different concentrations of n-hexane.

[0055] Divide the inorganic salt culture solution into 300mL shake flasks each with a volume of 50mL, and sterilize at 110°C for 40min. After the sterilization, it was placed at room temperature for 2 days to confirm that there was no growth of miscellaneous bacteria. Add the quiescent cells that the final concentration reaches 50mg / L (with cell dry weight) embodiment 2 method acquisition, then add n-hexane as the only carbon source to make the final concentration respectively 85, 195, 230, 335, 405mg / L, After the shaker flask was sealed, culture it on a shaker at 30°C and 160 rpm, and make a blank control without adding bacteria. Regularly measure the concentration of n-hexane remaining in the shake flask, and draw the removal rate curve of different initial concentrations of n-hexane over time for the strains, the results are shown in Figu...

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Abstract

The invention discloses Mycolicibacteriumneworleansense WCJ and application thereof in degradation of organic pollutants. According to the application, the Mycolicibacteriumneworleansense WCJ is inoculated into an inorganic salt culture solution containing organic pollutants and having the pH value of 4-9, and culture is performed at 25-35 DEG C to realize degradation of the organic pollutants. The Mycolicibacteriumneworleansense WCJ disclosed by the invention has an efficient degradation effect on n-hexane, and the pollutants can be more completely converted into harmless substances such as CO2, H2O and the like; and meanwhile, the strain can degrade other common industrial pollutants such as butyl acetate, petroleum ether, ethanol and the like to different degrees, so that the strain has a wide application prospect in biological purification of industrial waste gas and wastewater.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain-mycobacterium new orleans WCJ and its application in degrading organic pollutants. (2) Background technology [0002] n-Hexane is an organic compound, which belongs to straight-chain saturated aliphatic hydrocarbons, obtained by cracking and fractionating crude oil, and is a colorless liquid with a faint special smell. It is volatile, almost insoluble in water, and easily soluble in chloroform, ether, and ethanol. It is mainly used as a solvent, such as vegetable oil extraction solvent, propylene polymerization solvent, rubber and paint solvent, and pigment thinner. It is used for the extraction of soybean, rice bran, cottonseed and other edible oils and oils in spices. In addition, n-hexane isomerization is one of the important processes for producing high-octane gasoline blending components. [0003] Although n-hexane is of low toxicity, it is considered a high-risk poison because of its high vol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72C12R1/32C02F101/32C02F101/34
CPCC12N1/20C02F3/34B01D53/84B01D53/72C02F2101/32C02F2101/34Y02A50/20Y02P20/59
Inventor 成卓韦王晨洁陈建孟王家德张士汉赵景开
Owner ZHEJIANG UNIV OF TECH
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