Solid-phase reaction vessel and measurement method using same

Abstract

The invention provides a solid-phase reaction container using a biological specific reaction and a measurement method using the solid-phase reaction container. The solid-phase reaction container can realize extremely small amount of sample liquid and rapid measurement, and can provide stable measurement precision. A solid-phase reaction vessel and a measurement method using the same, the solid-phase reaction vessel being provided with: an upper cover member having a conical opening in a substantially central portion of a top surface portion; a lower cover member formed so as to be capable of fitting with the upper cover member and having a ventilation portion at the bottom; a first filter member that captures a substance to be detected contained in the sample liquid supplied through the opening; and a liquid suction member that is accommodated in an internal space formed by fitting the upper cover member and the lower cover member, and that sucks the sample liquid that has passed through the first filter. A second filter member is provided between the first filter member and the liquid-absorbing member, and the second filter member has a surface having wettability different from that of the surface of the liquid-absorbing member.

Description

technical field [0001] The present invention relates to a solid phase reaction container and an assay method using the solid phase reaction container. Background technique [0002] It has been known to use biologically specific reactions such as antigen-antibody reaction, sugar (chain)-lectin reaction, and biotin-avidin reaction to capture the detected substance contained in the sample on a porous substrate. Among them, the method of completing the above-mentioned reaction on a solid support is preferable from the viewpoint of the simplicity of the measurement. [0003] The measurement method using a solid phase support such as a porous matrix works synergistically with the concentration effect on the solid phase support to detect the analyte contained in the sample liquid only at an ultra-low concentration of nanograms. For example, in Patent Document 1, as the determination The step describes that a complex composed of a ligand-bound first physiologically active substance...

AI Technical Summary

Problems solved by technology

Especially in medical institutions where subjects suspected of having allergic diseases are diagnosed, in the case of identifying the allergen as the causative substance, it is necessary to provide a very small amount in consideration of the physical burden and time burden of the subject, etc. The rapid diagnostic material of the sample fluid, the existing technology is not satisfactory

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