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A group of antibody compositions for immunophenotyping of myeloid tumors and their applications

An antibody composition, immunophenotyping technology, applied in the direction of immunoglobulin, anti-animal/human immunoglobulin, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, etc. It is difficult to identify and affect the judgment of results, etc., to achieve the effect of reducing the number of needs and operations, increasing the number of uses, and saving operation time.

Active Publication Date: 2022-07-01
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in some MDS specimens, due to degranulation of granulocytes, mature granulocytes in the CD45 / SSC map, SSC is reduced and located in the position of monocytes, which is difficult to distinguish from monocytes, which seriously affects the results. judgment

Method used

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  • A group of antibody compositions for immunophenotyping of myeloid tumors and their applications
  • A group of antibody compositions for immunophenotyping of myeloid tumors and their applications
  • A group of antibody compositions for immunophenotyping of myeloid tumors and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Preparation of reagents

[0065] The present invention provides antibody combinations for three indications:

[0066] The first antibody combination, a total of 24 antibodies and 23 colors of fluorescein, was prepared according to the myeloid tumor combination in Table 4. The above antibodies were mixed and packed in a container respectively, and used for immunophenotyping markers of all specimens determined to be myeloid tumors (AML and chronic myeloid diseases).

[0067] The second antibody combination, a total of 22 kinds of antibodies and 21 colors of fluorescein, was prepared according to the AML combination in Table 4. Anti-CD105-SB436 and anti-CD10-BV711 were not added. The rest were the same as the myeloid tumor group, which were mixed separately. In 1 container, for immunophenotyping markers for specimens determined to be AML.

[0068] The third antibody combination, a total of 22 antibodies and 22 colors of fluorescein, was prepared according to th...

Embodiment 222-24

[0071] Example 2 Immunophenotype analysis of myeloid tumors by flow cytometric analysis of 22-24 antibody combinations

[0072] 1. Main experimental materials and instruments

[0073] 1. Materials: 10×PBS buffer, hemolysin for flow cytometer (BD company);

[0074] 2. Instrument: CytekNL-3000 model full spectrum flow cytometer, equipped with 405nm, 488nm, 635nm three lasers, 38 fluorescence detectors. Tabletop low-speed centrifuge, vortex mixer.

[0075] 2. Methods

[0076] 1. Sample collection:

[0077] Immediately place 1-3 mL of the collected human bone marrow fluid in a heparin anticoagulant tube and invert it several times to prevent the specimen from coagulating. Various cells such as pleural and ascites fluid and lavage fluid should be sent to the laboratory as soon as possible after collection. Refrigerate at 4°C. The flow cytometry (FCM) detection must be completed within 48 hours, and the operation should be performed according to the instructions.

[0078] 2. S...

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Abstract

The present invention relates to the field of antibody medicine, in particular to a group of antibody compositions for immunophenotyping of myeloid tumors and applications thereof, wherein the antibody compositions comprise a group of 22-24 antibody combinations. The invention optimizes the antibody combination and the corresponding antibody fluorescent labeling combination and the result interpretation method, and only needs to use a tube of 22 or 24 kinds of antibodies and a tube of cells to be sampled at one time, which can comprehensively and efficiently carry out subclassification of AML and chronic myeloid tumors. It can predict CML and some recurrent genetically abnormal AML, and has a high sensitivity for the diagnosis of MDS. At the same time, the leukemia-associated immunophenotype (LAIP) that can be used for post-treatment minimal residual disease (MRD) monitoring in this combination can be determined.

Description

technical field [0001] The present invention relates to the field of antibody medicine, in particular to a group of antibody compositions for immunophenotyping of myeloid tumors and applications thereof. Background technique [0002] Acute and chronic myeloid tumors are a large part of blood / lymphatic system tumors. The classification of acute myeloid tumors in the 2017 WHO classification of blood and lymphatic system tumors includes acute myeloid leukemia and related myeloid precursor cells. Tumors, see Table 1, are divided into 7 categories. As can be seen from the WHO classification, the diagnosis of blood / lymphatic system tumors highlights the important roles of morphological / pathological (M), immunophenotyping (I), genetics (C) and gene (M) testing. . [0003] In the classification of AML, the first type of AML with recurrent genetic abnormalities is based on cytogenetics and genes as the final diagnosis. However, some of the subtypes have characteristic immunophenot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28G01N33/577G01N33/574G01N15/14
CPCC07K16/2866C07K16/2896C07K16/289C07K16/283C07K16/2833C07K16/2803C07K16/2812C07K16/2845C07K16/2848C07K16/2881G01N33/577G01N33/57426G01N33/56972G01N15/14G01N2800/60G01N33/57492
Inventor 刘艳荣王亚哲
Owner PEOPLES HOSPITAL PEKING UNIV
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