Method for extracting anthocyanidin from petals of Nanthorne mustard

A technology of anthocyanins and Arabidopsis thaliana, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problem of low purity of anthocyanins in Arabidopsis thaliana, great influence on the stability of anthocyanins, and difficulty in maintaining Bright colors and other issues, to achieve the effect that is conducive to industrial applications, bright colors, mild conditions

Pending Publication Date: 2022-03-25
WANHUA CHEM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors have found that the purity of the anthocyanins obtained through conventional extraction and purification is not high, and the stability of anthocyanins is greatly affected by the external environment, and color changes are likely to occur during the extraction process. , it is difficult to keep the original color bright
[0003] At present, there is no research report on the extraction of anthocyanins with high purity and stable properties from the petals of Arabidopsis thaliana

Method used

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  • Method for extracting anthocyanidin from petals of Nanthorne mustard
  • Method for extracting anthocyanidin from petals of Nanthorne mustard

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Pick fresh purple Arabidopsis flowers, remove flower peduncles, stamens and other parts, select purple petals, wash 100g of fresh petals, mash them into mud with a mortar, add 300g of ethanol with a volume ratio of 1.5:1- Aqueous solution (added with ascorbic acid to adjust the pH of the solution to 2), soaked for 20 minutes, and filtered to obtain the filtrate; the remaining filter residue was soaked and filtered repeatedly until the color of the filtrate became lighter, and the combined filtrate was used as the extract.

[0039] The extract was subjected to vacuum extraction at 42° C. and 0.1 MPa for 90 minutes to obtain anthocyanin-containing aqueous solution 1 . Fill the column with 55mL XAD-7 macroporous adsorption resin with a height-to-diameter ratio of 4:1, and pass through the column with 3BV absolute ethanol at a flow rate of 1BV / h to clean the organic residue in the resin. Clean resin to TOC (total organic carbon) below 5ppm.

[0040] The above-mentioned ext...

Embodiment 2

[0043] Pick fresh purple Arabidopsis flowers, remove flower stalks, stamens and other parts, choose purple petals, wash 100g of fresh petals, mash them into mud with a mortar, add 300g of ethanol with a volume ratio of 4:1- Aqueous solution (ascorbic acid was added to adjust the pH of the solution to be 3), soaked for 20 minutes, and then filtered to obtain the filtrate; the remaining filter residue was soaked and filtered repeatedly until the color of the filtrate became lighter, and the combined filtrate was used as the extraction solution.

[0044] The extraction solution was subjected to vacuum extraction at 45° C. and 0.2 MPa for 120 minutes to obtain anthocyanin-containing aqueous solution 1 . Fill the column with 55mL AB-8 macroporous adsorption resin with a height-to-diameter ratio of 4:1, and pass through the column with 3BV absolute ethanol at a flow rate of 1BV / h to clean the organic residue in the resin. After alcohol washing, use deionized water Clean resin to TOC...

Embodiment 3

[0048] Pick fresh purple Arabidopsis flowers, remove flower peduncles, stamens and other parts, select purple petals, wash 100g of fresh petals, mash them into mud with a mortar, add 300g of ethanol with a volume ratio of 2.3:1- Aqueous solution (add ascorbic acid to adjust the pH of the solution to 1), soak for 20 minutes, and then filter to obtain the filtrate; repeat the above soaking and filtering operations for the remaining filter residue until the color of the filtrate becomes lighter, and combine the filtrates as the extraction solution.

[0049] The extract was subjected to vacuum extraction at 45° C. and 0.2 MPa for 100 minutes to obtain anthocyanin-containing aqueous solution 1 . 55mL The macroporous adsorption resin is packed into the column with a height-to-diameter ratio of 4:1, and 3BV of absolute ethanol is used to pass through the column at a flow rate of 1BV / h to clean the organic residue in the resin. After the alcohol washing, the resin is cleaned with dei...

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Abstract

The method comprises the following steps: (1) washing and mashing the petals of the Nanzhou mustard, putting the petals of the Nanzhou mustard into a water-containing extracting agent for soaking treatment, and filtering to obtain an extracting solution; 2) carrying out reduced pressure extraction on the leach liquor to obtain an aqueous solution 1 containing anthocyanin; (3) carrying out adsorption treatment on the aqueous solution 1 containing the anthocyanin by adopting macroporous adsorption resin, and then desorbing to obtain eluent enriched with the anthocyanin; the method comprises the following steps of (1) extracting an eluent, (2) carrying out reduced pressure extraction on the eluent to obtain an aqueous solution 2 containing anthocyanin, (5) adding a composite extractant into the aqueous solution 2 containing anthocyanin for extraction, removing an organic phase and collecting a water phase, and (6) freeze-drying the water phase obtained by extraction to obtain a powdery anthocyanin product.The method can be used for preparing the anthocyanin product with high purity and good stability.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins from Arabidopsis thaliana petals. Background technique [0002] Arabidopsis thaliana is a plant of the Brassicaceae Arabidopsis genus, and its flowers are mostly rich purple. Its color is gorgeous and eye-catching, warm and refined, and the small flowers are densely crowded together, like a group of purple butterflies. The purple color of Arabidopsis thaliana comes from the anthocyanins in the petals. If the anthocyanins can be extracted, they can be used as natural pigments and have the advantage of being non-toxic and harmless. However, the inventors have found that the purity of the anthocyanins obtained through conventional extraction and purification is not high, and the stability of anthocyanins is greatly affected by the external environment, and color changes are likely to occur during the extraction process. , it is difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 袁雪欧洋赵伟国孙家宽
Owner WANHUA CHEM GRP CO LTD
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