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Near-infrared II region nanoprobe for liver imaging and preparation and application thereof

A nano-probe and near-infrared technology, applied in the field of bioluminescent analysis, can solve the problems of less attention to the diagnosis of liver tissue diseases, no obvious clinical symptoms, and difficulty in diagnosing acute alcoholic liver injury, and achieve the effect of improving sensitivity

Active Publication Date: 2022-03-25
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, the NIR-II probes in the prior art often focus on the diagnosis of tumor-related diseases, and less attention is paid to the diagnosis of liver tissue diseases, and the probe imaging research and diagnosis of acute alcoholic liver injury are even more important. very few
And because acute alcoholic liver injury usually has no obvious clinical symptoms, it is difficult to diagnose patients with acute alcoholic liver injury using the NIR-II probe of the prior art

Method used

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  • Near-infrared II region nanoprobe for liver imaging and preparation and application thereof
  • Near-infrared II region nanoprobe for liver imaging and preparation and application thereof
  • Near-infrared II region nanoprobe for liver imaging and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation route of a nano-probe in the near-infrared region II that can be used for the diagnosis of acute alcoholic liver injury and the evaluation of the curative effect is shown in the following reaction formula:

[0043]

[0044] in: Represents TPB that satisfies the general formula (the same below), that is, 4,8-bis[4-(N,N-diphenylamino)phenyl]benzo[1,2-c:4,5-c]bis [1,2,5]thiadiazole; the dotted line segment in DSPE-PEG2000 represents PEG, and the solid line segment represents DSPE; in the product NTPB-NPs, TPB is connected by DSPE to form an assembled structure, and PEG is chain-like and distributed in the assembled outside of the structure.

[0045] Specifically, 3 mg TPB and 15 mg DSPE-PEG-2000 were dissolved in 3 mL THF, and then 27 mL deionized water was added. Vortexed for 1 min at 25 °C and the resulting mixture was purged in a water bath for 24 h using a dialysis bag (3500 Da). Centrifuge at 8000rpm for 5 minutes to remove large particles and ins...

Embodiment 2

[0047] A preparation route of a nano-probe in the near-infrared region II that can be used for the diagnosis of acute alcoholic liver injury and the evaluation of the curative effect is shown in the following reaction formula:

[0048]

[0049] Specifically, 3 mg of TPB and 15 mg of DSPE-PEG-2500 were dissolved in 3 mL of DMSO, and then 27 mL of deionized water was added. Vortexed for 1 min at 50 °C and the resulting mixture was purged in a water bath for 12 h using a dialysis bag (2500 Da). Centrifuge at 8000rpm for 5 minutes to remove large particles and insoluble material. Before use, the NTPB NPs solution was freeze-dried or concentrated by ultrafiltration. By measuring the concentration of free TPB in absorbance analysis using a pre-established calibration curve, the encapsulation efficiency of NTPB NPs was calculated to be approximately 58%.

Embodiment 3

[0051] A preparation route of a nano-probe in the near-infrared region II that can be used for the diagnosis of acute alcoholic liver injury and the evaluation of the curative effect is shown in the following reaction formula:

[0052]

[0053] Specifically: Dissolve 3mg TPB and 15mg DSPE-PEG-3000 in 3mL CH 3CN, then add 27 mL of deionized water. Vortexed for 1 min at 40 °C, the resulting mixture was purged in a water bath for 36 h using a dialysis bag (2000 Da). Centrifuge at 8000rpm for 5 minutes to remove large particles and insoluble material. Before use, the NTPB NPs solution was freeze-dried or concentrated by ultrafiltration. By measuring the concentration of free TPB in absorbance analysis using a pre-established calibration curve, the encapsulation efficiency of NTPB NPs was calculated to be approximately 64%.

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Abstract

The invention belongs to the technical field of biological fluorescence analysis, and discloses a near-infrared II region nanoprobe for liver imaging and preparation and application thereof, the near-infrared II region nanoprobe comprises a TPB component, a polyethylene glycol PEG component and a distearoyl phosphatidyl ethanolamine DSPE component, the TPB components are connected through the distearoyl phosphatidyl ethanolamine DSPE component to form an assembled structure; the polyethylene glycol PEG component is in a chain shape and is distributed on the outer side of the assembled structure. The probe structure is improved, a new fluorophore and an amphoteric material are adopted to design and synthesize the near-infrared II-region fluorescent probe, and the near-infrared II-region fluorescent probe is constructed for the fluorophore based on a TPB structure, so that the acute alcoholic liver injury liver tissue can be detected, and the curative effect of the medicine for treating the acute alcoholic liver injury can be further evaluated.

Description

technical field [0001] The invention belongs to the technical field of bioluminescence analysis, and more specifically relates to a near-infrared region II nanoprobe used for liver imaging and its preparation and application, which can be used for diagnosing acute alcoholic liver injury and treating acute alcoholic liver injury Evaluating the efficacy of medication, etc. Background technique [0002] Acute alcoholic liver injury (AALI) is an early pathological feature of alcoholic liver disease (ALD). Without proper intervention, AALI may develop into liver fibrosis and even cirrhosis and hepatocellular carcinoma, leading to a series of complications such as hepatic encephalopathy, liver failure and portal hypertension, thus becoming one of the leading causes of death in young people one. In addition, AALI usually has no obvious clinical symptoms, making it difficult for patients to diagnose. Therefore, finding effective methods to accurately diagnose AALI is of great imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N21/64A61K49/00
CPCC09K11/025C09K11/06A61K49/0054A61K49/0052A61K49/0021G01N21/6428C09K2211/1081C09K2211/1007C09K2211/1014
Inventor 王凯平聂刚张玉宋梦姿许静雅崔政汪会玲
Owner HUAZHONG UNIV OF SCI & TECH
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