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Salt-tolerant bacillus and application thereof

A technology of bacillus halide and inoculum, which is applied in the field of microorganisms, can solve the problem of not being able to effectively inhibit the biocontrol bacteria of holly japonica, and achieve the effects of not easily producing drug resistance, a simple preparation method, and good drug efficacy persistence.

Active Publication Date: 2022-03-25
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no biocontrol fungus capable of effectively inhibiting Calonectriailiicola, Septoria glycines, Phomopsis sojae and their corresponding diseases in the prior art.

Method used

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  • Salt-tolerant bacillus and application thereof
  • Salt-tolerant bacillus and application thereof
  • Salt-tolerant bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The acquisition and identification of embodiment 1 halotolerant bacillus BAH-1

[0071] 1. Screening and isolation of Bacillus halotolerant BAH-1 strain

[0072] (1) Sample collection: collect fresh leaves of soybean plants in the base of Cangzhou Academy of Agricultural Sciences, Hebei Province, rinse the surface with dust with sterile water, and then carry out surface disinfection of plant leaves (soak with 75% alcohol for 1 min and 8% Soak in NaClO for 4min to carry out surface disinfection treatment), wash with sterile water 4 times;

[0073] (2) Separation and screening: Cut the leaves into 1cm×1cm pieces, add water and grind them into a paste, let them stand for 10 minutes, spread them on LB plates containing 10g / L NaCl, and culture them at 28°C for 48 hours;

[0074] (3) Purification: After the culture grows out, use the plate streaking separation method to purify, pick the colonies that grow at the highest concentration and streak on the enrichment medium until t...

Embodiment 2

[0097] The preparation of the bacterium liquid of embodiment 2 halotolerant bacillus BAH-1 and bacterial agent

[0098] The preparation method of the bacterium liquid and the bacterium agent of halotolerant bacillus BAH-1 comprises the following steps:

[0099] (1) Strain activation: pick the Bacillus halotolerant BAH-1 strain to the strain preservation medium, streak and pick a single colony for two times at 28°C, then pick a single colony in the strain activation medium, Incubate at 30°C for 30 h on a shaker at 180 r / min;

[0100] (2) Preparation of liquid seeds: in a fermenter equipped with a high-temperature sterilized seed medium, inoculate the BAH-1 activated bacterial liquid obtained in step (1) according to an inoculum size of 10% (V / V), at 30° C. , cultivated in air for 18 hours to obtain liquid seeds;

[0101] (3) liquid state fermentation: in the seed culture medium that is equipped with through high temperature sterilization, inoculate liquid seed according to th...

Embodiment 3

[0103] The bacteriostasis of embodiment 3 halotolerant bacillus BAH-1

[0104] The present embodiment analyzes the inhibitory action of Bacillus halotolerant BAH-1 to Calonectriailiicola, Septoria glycines and Phomopsis sojae of soybean. The specific methods are as follows:

[0105] (1) Inoculate the pathogenic bacteria of Calonectria ilicicola, Septoriaglycines and Phomopsis sojae of soybean on the PDA plate respectively, and Calonectria ilicicola (Calonectria ilicicola) was inoculated at 28 Cultivate Septoria glycines and Phomopsis sojae at 25°C. After culturing to cover the plate respectively, use a puncher with a diameter of 5 mm to make a fungus cake. The cake was inoculated in the center of the PDA plate, and at the same time, the BAH-1 bacterial solution (concentration 10 8 cfu / mL, 20 μ L), with the BAH-1 bacterium liquid prepared in embodiment 2 as experimental group, simultaneously with the plate that only inoculates pathogenic fungus as control group, each treatment...

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Abstract

The invention relates to the technical field of microorganisms, in particular to salt-tolerant bacillus and application thereof. The salt-tolerant bacillus strain BAH-1 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain BAH-1 is CGMCC No. 23503. The halotolerant bacillus BAH-1 is high in colonization capacity and breeding speed in plants, has the effect of efficiently inhibiting plant pathogenic bacteria, has the effect of simultaneously treating plant diseases such as red crown rot, brown spot and stem black spot disease, is high in control effect, has a very good application prospect in plant disease control, and has a wide application prospect. And a foundation is laid for the development of efficient and broad-spectrum biocontrol preparations.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a halotolerant bacillus strain and its application. Background technique [0002] Plant diseases are one of the important factors affecting plant growth and yield. At present, the use of chemical pesticides is still one of the main means of plant disease control. Although the use of chemical pesticides can effectively prevent and control the harm of pathogenic bacteria to crops, their high toxicity and serious residues will cause the bacteria to develop resistance, endanger human health, and cause damage to the surrounding environment. Biological control avoids a series of environmental problems caused by chemical pesticides, and is safer, more effective and durable. It has become a very important and effective measure in plant control and is increasingly valued. Biological control is to reduce the number of pathogenic bacteria or weaken the pathogenic activity of pathog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22Y02A40/10
Inventor 毕秋艳高占林党志红赵建江路粉吴杰郭江龙丁海波韩秀英王文桥
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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