Myocardial infarction related chromosome outer circular DNA as well as screening method and application thereof
A kind of myocardial infarction and extrachromosomal technology, applied in the field of biomedicine, can solve problems such as unreported
Patent Information
- Authority / Receiving Office
- CN · China
- Current Assignee / Owner
- Publication Date
- 2022-03-25
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Abstract
Description
technical field
[0001] The invention belongs to the technical field of biomedicine, and relates to extrachromosomal circular DNA related to myocardial infarction and its screening method and application. Background technique
[0002] Extrachromosomal circular DNA (eccDNA) widely exists in various eukaryotic organisms including humans, and is closely related to the occurrence and development of many diseases. Sin STK et al. (Sin STK, JiangP, Deng J, et al. Identification and characterization of extrachromosomal circular DNA in maternal plasma. Proc Natl Acad Sci U S A. 2020; 117(3): 1658-1665. doi: 10.1073 / pnas. 1914949117) showed that maternal and fetal cell-free circular DNA can be isolated from plasma of pregnant women, Sun Z et al. (Sun Z, Ji N, Zhao R, Liang J, Jiang J, TianH.Extrachromosomal circular DNAs are common and functional in esophageal squamous cell carcinoma.Ann Transl Med.2021;9(18):1464.doi:10.21037 / atm-21-4372) revealed the eccDNA expression profile in eso...
Examples
Embodiment
[0030] Concrete implementation process of the present invention is as follows:
[0031] 1. Sample collection and processing
[0032] The samples of the myocardial infarction group were all collected from inpatients in the Department of Cardiology, the Second Affiliated Hospital of Soochow University. The diagnosis of myocardial infarction was in accordance with the "2017 European Guidelines for the Diagnosis and Treatment of Acute ST-segment Elevation Myocardial Infarction". The samples of the control group were from the Second Affiliated Hospital of Soochow University. The peripheral blood of the healthy population in the physical examination center, the myocardial infarction group and the control group each include five subjects, all of which are male and younger than 35 years old.
[0033] Use an anticoagulant tube to collect whole blood, and centrifuge the plasma as soon as possible: centrifuge the sample at 3000g at 4°C for 10 minutes to separate the plasma and cell compo...