A reporter gene system for screening biofilm inhibitors

Abstract

The present invention relates to a novel reporter gene system for screening biofilm inhibitors. The application proves that the bsrA gene can inhibit the formation of Pseudomonas aeruginosa biofilm, and its coding gene is overexpressed in bacteria, which can significantly inhibit the formation of biofilm Yield. Therefore, using this gene as a marker, a new reporter gene system is constructed to screen small molecular compounds that can activate the expression of the marker gene, which can inhibit the synthesis of biofilm from the gene level and help to solve the problem caused by biofilm. Persistent infection, antibiotic resistance, and at the same time, by studying the mechanism of this gene to inhibit the formation of biofilm, we can better solve the problem of microbial biofilm formation mechanism and the inhibition and removal of biofilm in the food processing industry.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular, the invention relates to the application of a regulation gene in inhibiting microorganism biofilm (biofilm). By constructing a fusion reporter gene system to screen a small molecular compound to activate the screening marker gene, the expression of the marker gene is increased, thereby inhibiting the biofilm synthesis. It is of great significance for the multi-drug resistance caused by Pseudomonas aeruginosa and the food-borne diseases caused by biofilm. Background technique [0002] At present, the treatment of nosocomial infection mainly uses antibiotics to kill or inhibit the growth of pathogenic bacteria. However, Pseudomonas aeruginosa itself has some drug resistance mechanisms, including the outer membrane with low permeability, the expression of constitutive genes of different efflux pumps, and the production of antibiotic inactivating enzymes (such as cephalosporinase), etc.,...

AI Technical Summary

Problems solved by technology

The formation of biofilms has a serious impact on industrial environments. On the one hand, biofilms can cause a series of problems in industrial operations, such as reduced heat transfer, clogged pipes, clogged filters, and cause surface damage.

On the other hand, biofilm is very easy to form a pollution source, especially in the food industry, where bacteria adhere to food contact surfaces, providing an extremely rich source of pathogen pollution for bacterial contamination, and bringing inevitable risks to human health

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