High performance liquid chromatography detection method for specific chromatogram of honey-fried licorice root decoction pieces

A high-performance liquid chromatography and inspection method technology, which is applied in the field of medicinal material inspection, can solve the problems of unstable frontal area, unstable retention time of medicinal materials and decoction pieces of Zhigan grassland, unstable chromatographic conditions, etc., and achieves the effect of a reliable detection method.

Pending Publication Date: 2022-04-12
陕西孙思邈高新制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the inspection standard of Zhigancao decoction pieces is "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of Zhigancao grassland medicinal materials and decoction pieces is unstable, the front area is unstable, and a series of chromatographic conditions such as front state and main peak Unstable, therefore, we propose a high performance liquid chromatography method for the characteristic map of Zhilicorice decoction pieces

Method used

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  • High performance liquid chromatography detection method for specific chromatogram of honey-fried licorice root decoction pieces
  • High performance liquid chromatography detection method for specific chromatogram of honey-fried licorice root decoction pieces
  • High performance liquid chromatography detection method for specific chromatogram of honey-fried licorice root decoction pieces

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Experimental program
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Effect test

Embodiment 1

[0027] see figure 1 Shown: the present invention is a high-performance liquid chromatography test method for the characteristic spectrum of Radix Glycyrrhiza Radix Glycyrrhizae, wherein, it includes mobile phase: acetonitrile is used as mobile phase A, 0.1% phosphoric acid solution is used as mobile phase B, and gradient elution is carried out according to the following table.

[0028]

[0029] Flow rate: 0.7ml / min

[0030] Detection wavelength: 237nm

[0031] Column temperature: 30°C

[0032] The retention time graph of liquiritin in the reference substance and liquiritin in the test product and methanol solvent, the number of theoretical plates is greater than or equal to 5000.

[0033] Solution preparation

[0034] Preparation of reference solution

[0035] Take 0.2g of licorice (licorice) reference medicinal material, put it in a stoppered Erlenmeyer flask, add 100ml of 70% ethanol, seal it tightly, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let coo...

Embodiment 2

[0041] Glycyrrhiza licorice has chosen the current domestic mainstream production areas, namely Inner Mongolia Etuokeqian Banner, Ningxia Tongxin, Ningxia Yanchi, Xinjiang Ural, Gansu Wuwei Minqin County, a total of 5 production areas, and each production area chooses 3 suppliers and 1 each ~2 batches, a total of 18 batches were studied.

[0042] Inspection results of 18 batches of roasted licorice

[0043] Relative retention time of S1 in 18 batches of Zhigancao decoction pieces

[0044] Relative retention time in 18 batches of Radix Paeoniae Alba

[0045]

[0046]

[0047] Relative retention time of S2 in 18 batches of Zhigancao decoction pieces

[0048] Relative retention time in 18 batches of Radix Paeoniae Alba

[0049]

[0050]

[0051] Relative retention time of S3 in 18 batches of Zhigancao decoction pieces

[0052] Relative retention time in 18 batches of Radix Paeoniae Alba

[0053]

[0054]

[0055] The above proves that the embodiment is feas...

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Abstract

The invention discloses a high performance liquid chromatography detection method for specific chromatograms of honey-fried licorice root decoction pieces, and relates to the technical field of medicinal material detection. The detection method adopts acetonitrile as a mobile phase A and a 0.1% phosphoric acid solution as a mobile phase B, and comprises the following elution steps: step S1, gradient elution time is 0-9 min, the mobile phase A accounts for 19-22%, and the mobile phase B accounts for 81-78%; s2, gradient elution time is 9-18 min, the mobile phase A accounts for 22%-30%, and the mobile phase B accounts for 78%-70%; s3, gradient elution is carried out for 18-29 min, the mobile phase A accounts for 30%-66%, and the mobile phase B accounts for 70%-34%; s4, gradient elution is carried out for 29-33 min, the mobile phase A accounts for 66-19%, and the mobile phase B accounts for 34-81%; s5, gradient elution is carried out for 33-45 min, the mobile phase A accounts for 19%, and the mobile phase B accounts for 81%. The high performance liquid chromatography test method for the specific chromatogram of the honey-fried licorice root decoction piece makes up the vacancy that no test method for the specific chromatogram of the honey-fried licorice root decoction piece exists at present.

Description

technical field [0001] The invention relates to the technical field of medicinal material inspection, in particular to a high-performance liquid chromatography inspection method for characteristic maps of roasted licorice slices. Background technique [0002] Glycyrrhiza licorice is a round or oval slice, the surface is reddish brown or grayish brown, slightly shiny, the cut surface is yellow to dark yellow, the cambium ring is obvious, and the rays are radial. Glycyrrhiza licorice is a traditional Chinese medicine decoction piece in China, which has the function of nourishing the spleen and stomach , the effect of replenishing qi and rejuvenating the pulse is widely used in clinical practice, and the dosage is extremely large; [0003] At present, the inspection standard of Zhigancao decoction pieces is "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of Zhigancao grassland medicinal materials and decoction piece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
Inventor 王光磊何亮张乔
Owner 陕西孙思邈高新制药有限公司
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