High performance liquid chromatography detection method for specific chromatogram of honey-fried licorice root decoction pieces

Abstract

The invention discloses a high performance liquid chromatography detection method for specific chromatograms of honey-fried licorice root decoction pieces, and relates to the technical field of medicinal material detection. The detection method adopts acetonitrile as a mobile phase A and a 0.1% phosphoric acid solution as a mobile phase B, and comprises the following elution steps: step S1, gradient elution time is 0-9 min, the mobile phase A accounts for 19-22%, and the mobile phase B accounts for 81-78%; s2, gradient elution time is 9-18 min, the mobile phase A accounts for 22%-30%, and the mobile phase B accounts for 78%-70%; s3, gradient elution is carried out for 18-29 min, the mobile phase A accounts for 30%-66%, and the mobile phase B accounts for 70%-34%; s4, gradient elution is carried out for 29-33 min, the mobile phase A accounts for 66-19%, and the mobile phase B accounts for 34-81%; s5, gradient elution is carried out for 33-45 min, the mobile phase A accounts for 19%, and the mobile phase B accounts for 81%. The high performance liquid chromatography test method for the specific chromatogram of the honey-fried licorice root decoction piece makes up the vacancy that no test method for the specific chromatogram of the honey-fried licorice root decoction piece exists at present.

Description

technical field [0001] The invention relates to the technical field of medicinal material inspection, in particular to a high-performance liquid chromatography inspection method for characteristic maps of roasted licorice slices. Background technique [0002] Glycyrrhiza licorice is a round or oval slice, the surface is reddish brown or grayish brown, slightly shiny, the cut surface is yellow to dark yellow, the cambium ring is obvious, and the rays are radial. Glycyrrhiza licorice is a traditional Chinese medicine decoction piece in China, which has the function of nourishing the spleen and stomach , the effect of replenishing qi and rejuvenating the pulse is widely used in clinical practice, and the dosage is extremely large; [0003] At present, the inspection standard of Zhigancao decoction pieces is "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of Zhigancao grassland medicinal materials and decoction piece...

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Problems solved by technology

[0003] At present, the inspection standard of Zhigancao decoction pieces is "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of Zhigancao grassland medicinal materials and decoction pieces is unstable, the front area is unstable, and a series of chromatographic conditions such as front state and main peak Unstable, therefore, we propose a high performance liquid chromatography method for the characteristic map of Zhilicorice decoction pieces

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