Adrenocorticotrophic hormone assay kit and preparation method thereof

An adrenal cortex and kit technology, applied in the field of immunoassay, can solve the problems affecting the accuracy of results, poor stability of reagents, magnetic bead agglutination, etc., and achieve the effects of reliable detection means, good stability and strong specificity

Inactive Publication Date: 2019-01-18
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, direct coating of antibodies to magnetic beads is likely to cause agglutination of the magnetic beads, resulting in poor stability of the reagent and affecting the accuracy of the detection results.

Method used

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  • Adrenocorticotrophic hormone assay kit and preparation method thereof
  • Adrenocorticotrophic hormone assay kit and preparation method thereof
  • Adrenocorticotrophic hormone assay kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1 calibrator, quality control product

[0051] Take 1ml of 0.4μg / mL corticotropin stock solution, add it to 99ml calibrator buffer solution, and dilute to 4000pg / mL calibrator stock solution. Then dilute the calibrator mother solution in sequence to the concentration points of 2000pg / mL, 1000pg / mL, 200pg / mL, 50pg / mL, 10pg / mL, 1pg / mL, and use the calibrator matrix buffer as the concentration point of 0pg / mL point. The composition of the calibrator buffer: composed of 0.1mol / L PB, 0.15mol / L sodium chloride, 1% bovine serum albumin and 0.05% tween-20, pH value 6.5, stored at 2-8°C for later use .

[0052] Add 10ml of calibrator mother solution to 30ml of calibrator matrix buffer to prepare 40ml of high-concentration quality control with a concentration of 1000pg / mL.

[0053] Add 0.5ml of calibrator mother solution to 39.5ml calibrator matrix buffer solution to prepare 40ml of low-concentration quality control substance with a concentration ...

Embodiment 2

[0054] The preparation of embodiment 2 R1

[0055] Take the streptavidin magnetic particle solution with a concentration of 100 mg / ml, add 20 times the volume of TBST solution and mix well for 10 minutes, then place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, and keep the magnetic Particles: After repeated washing 3 times, the solid-phase reagent R1 with a magnetic bead concentration of 0.03% was prepared with 50mM MES buffer solution containing 0.05% Tween-20 and 0.05% Proclin300, pH 6.5.

Embodiment 3

[0056] The preparation of embodiment 3 R1

[0057] Take the streptavidin magnetic particle solution with a concentration of 50 mg / ml, add 5 times the volume of TBST solution and mix well for 15 minutes, then place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, and keep the magnetic Particles: after repeated washing 3 times, use 50mM MES buffer solution containing 0.05% Tween-20 and 0.05% Proclin300, pH 6.5 to prepare solid-phase reagent R1 with a magnetic bead concentration of 0.1%.

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Abstract

The invention discloses an adrenocorticotrophic hormone assay kit and a preparation method thereof, and belongs to the technical field of immunoassay. The problem that the test accuracy in the prior art is bad is solved. The kit comprises the following reagents: R1, suspension containing streptavidin-conjugated magnetic nanoparticles; R2, the suspension containing adrenocorticotrophic hormone marked by a chemoluminescence marker; R3, the susnepsion containing the adrenocorticotrophic hormone marked by biotin, and a calibration product and a quality control product. The adrenocorticotrophic hormone assay kit prepared by adopting the method disclosed by the invention has relatively high detection sensitivity, and is simple in operation, accurate and fat in quantitative detection, and can beextensively applied to the clinical detection of the adrenocorticotrophic hormone.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to a corticotropin assay kit and a preparation method thereof. Background technique [0002] Adrenocorticotropic hormone (ACTH), a thirty nine peptide, can promote the hyperplasia of adrenal cortex and the generation and secretion of adrenal cortex hormone. ACTH prepared from sharks, frogs, ostriches, and mammalian pituitary glands are all thirty nine peptides. Amino acid residues 1-24 and 34-39 of ACTH of different species of animals are basically the same, and the obvious structural differences reflect In the carboxy-terminal region of the molecule (position 25-33). The sequence of amino acids in the amino-terminal part (positions 1 to 24) is relatively consistent, and it is the central area of ​​biological activity. ACTH and tetrapeptides in its amino-terminal region have been artificially synthesized, and its physiological activity is the same as that of natural ACTH. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/58G01N33/543G01N33/553G01N33/532
Inventor 胡雪凇唐仁陶高阳崔秀雲高威孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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