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Microbial culture medium and separation method of Rujgera

A technology of microbial culture medium and separation method, which is applied in the field of microbial culture medium and the isolation of Rugeria, and can solve the problems of low efficiency of the separation of Rugeria

Pending Publication Date: 2022-04-22
SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of this application is to provide a microbial culture medium and a method for isolating Rugeria, aiming to solve the technical problem of low efficiency of the existing Rugeria isolation

Method used

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  • Microbial culture medium and separation method of Rujgera
  • Microbial culture medium and separation method of Rujgera
  • Microbial culture medium and separation method of Rujgera

Examples

Experimental program
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Effect test

Embodiment 1

[0046] (1) Treatment of different components of coral samples

[0047] 1) Take a coral block with a diameter of 2-8cm, seal it in a sterilized ziplock bag under water, and wear it with a zipper bag. Take 500mL of in-situ seawater into a sampling bottle and package it underwater.

[0048] 2) Mucus: wash the coral block surface with sterile sea water three times to remove seawater pollution, in an ultra-clean workbench, put the washed coral block upside down in a sterile petri dish, place it under a low-speed sterile wind, and collect the coral surface Mucus, stored in a 2mL centrifuge tube to obtain coral mucus samples.

[0049] 3) Tissue: The coral block was transferred to sterile seawater Votex and washed three times, placed in a 50mL centrifuge tube and centrifuged at 4500rpm for 3 minutes until the mucus was removed (if the coral continues to secrete mucus and cannot be removed, use 2% sterile magnesium chloride The solution anesthetizes the coral block for 2 minutes, and...

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Abstract

The invention relates to the technical field of microbial separation, in particular to a microbial culture medium and a separation method of Rujgera. A nitrogen source of the microbial culture medium comprises at least one of betaine and choline, and N-acetylglucosamine. Experiments prove that the nitrogen source (including at least one of betaine and choline and N-acetylglucosamine) can be specifically and efficiently utilized as a culture substrate by the rugegeri, and efficient growth of the rugegeri can be realized, so that the microbial culture medium has a good application prospect in screening and separation of the rugegeri.

Description

technical field [0001] The application belongs to the technical field of microbial separation, and in particular relates to a microbial culture medium and a method for separating Rugeria spp. Background technique [0002] Ruegeria is a microorganism belonging to the genus Ruegeria, which has been found to have certain research value. There have been literature reports on the separation method of Rugeria spp., which uses taurine, which is abundant in coral mucus, as the carbon source and ammonium chloride as the nitrogen source in the medium. [0003] Taurine is a small-molecule organic acid that can be efficiently utilized by a variety of marine microorganisms, especially the major bacteria Roseobacter, including Rugeria, so taurine is used as a medium. The carbon source can enrich Roseobacter to a certain extent. Ammonium chloride or ammonium salts are nitrogen sources that can be widely used by microorganisms, and generally do not have screening effects. Therefore, alth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02
Inventor 罗海伟罗丹丽谢媚
Owner SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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