Application of litsea coreana extract in prevention and treatment of obesity and intestinal flora imbalance
A technology for intestinal flora imbalance and eagle tea, which is applied in the directions of food ingredients containing natural extracts, applications, medical preparations containing active ingredients, etc., to achieve the effects of convenient taking, simple extraction process, and easy prevention and treatment of obesity.
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[0027]Example: Obesity in mice induced by a high-fat and high-calorie diet
[0028] The method of free high-fat diet was used to establish a high-fat diet-induced obesity model in mice, and the prevention and treatment effect of eagle tea extract on mouse obesity was observed through animal model experiments.
[0029] 1. Experimental materials
[0030] 1.1 Experimental animals
[0031] SPF male C57BL / 6J mice, Animal Experiment Center, Chongqing Medical University.
[0032] 1.2 Extract
[0033] The eagle tea water extract is mainly extracted and prepared with pure water. The specific method is as follows:
[0034] Dry the raw material of eagle tea to be extracted, and crush it into small pieces; add pure water, the ratio of solid to liquid (g / mL) is 1:20, carry out reflux extraction, the extraction time is 2h, and obtain the extract; the extract Filtrate with a fiber membrane to obtain the filtrate and filter residue extracted for the first time; repeat the second extractio...
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