Effect of paeoniflorin in preparation of medicine for promoting survival of ischemic super-long random skin flap
A technology of paeoniflorin and medicine, which is applied in the field of action of medicine to achieve the effects of inhibiting cell pyroptosis, increasing the survival area ratio, and reducing inflammatory response
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Embodiment 1
[0020] Embodiment 1: the preparation method of paeoniflorin injection
[0021] Its preparation method steps are as follows
[0022] 1g of paeoniflorin powder was dissolved in 100ml of normal saline to make a 10mg / ml solution.
[0023] Wherein the chemical structural formula of paeoniflorin is as follows:
[0024]
Embodiment 2
[0025] Example 2: Establishment of random skin flap model of ischemia
[0026] 60 SD rats, provided by the Experimental Animal Center of Wenzhou Medical University, clean grade, weighing 200-250 g, aged 2-3 months. They were randomly divided into normal saline control group (control group), low-dose paeoniflorin group (Riv-L group) and high-dose group (Riv-H group), 20 rats in each group. Rats were anesthetized by intraperitoneal injection of 1% sodium pentobarbital (40mg / Kg), and the limbs and teeth were fixed in prone position. After the anesthesia took effect, the hair on the back trunk of the rat was preliminarily shaved with a shaver, the unremoved hair was moisturized with distilled water, and the depilatory cream was evenly applied. After waiting 3 minutes, gently remove the cream with a plastic sheet and repeat the process until the hair is completely removed. Design a modified McFarlane flap whose longitudinal axis is parallel to the long axis of the back of the rat...
Embodiment 3
[0028] Example 3: Detection of skin flap survival area ratio and skin flap survival status
[0029] Flap Survival Area Ratio and Flap Survival Condition Detection 1-7 days after operation, the color, texture, tissue elasticity, and hair growth or necrosis of rats were observed with the naked eye. Seven days after operation, the rats were anesthetized with 100 g / L chloral hydrate (8 mL / kg) and sacrificed. The surviving area of the flap and the total flap area of the rat were accurately traced and cut with transparent paper, and the mass was weighed with an electronic scale. Calculate the percentage of flap survival area [flap survival area transparent paper mass ÷ total flap surface area transparent paper mass × 100%]. Each rat was measured twice, and the average value was taken. The surviving area is pink, soft in texture, and has new hair growth; the necrotic area is black in color of the flap, the tissue retracts, has poor elasticity, and is hard in texture, and the cut...
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