High-pH antigen repair buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of high-pH antigen repair buffer solution

An immunohistochemical and buffer technology, applied in the field of automatic immunohistochemical staining machines, can solve problems such as affecting the effect of immunohistochemical staining, inability to soak tissue sections, and reducing the volume of repair solution, achieving good affinity, The effect of uniform distribution and improved recognition ability

Pending Publication Date: 2022-06-07
浙江莱阅病理诊断科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its own structural characteristics, the full-automatic staining instrument has a small container for the repair solution, and cannot completely immerse the tissue sections in the repair solution like manual repair, and the automatic immunohistochemical staining It needs to be carried out at a higher temperature (about 100°C), so the repair solution is easy to volatilize, further reducing the volume of the repair solution, making the repair effect relatively poor, and also seriously affecting the effect of subsequent immunohistochemical staining

Method used

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  • High-pH antigen repair buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of high-pH antigen repair buffer solution
  • High-pH antigen repair buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of high-pH antigen repair buffer solution

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Embodiment 1

[0027] A high-pH antigen retrieval buffer: every 1000 mL of the high-pH antigen retrieval buffer includes: 1.10 g of tris(hydroxymethyl) aminomethane (Tris), 0.31 g of ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), spit Warm-200.35mL, glycerol 150mL, ProClin9500.25mL and the balance of purified water, the pH range is pH8.9~9.1.

[0028]Preparation method: Weigh Tris and EDTA according to the proportion, dissolve Tris and EDTA with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClin950 after dissolution, and finally make up to volume with purified water. Rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the antigen retrieval buffer so that the pH value is in the range of pH 8.9 to 9.1.

Embodiment 2

[0030] A high-pH antigen retrieval buffer: every 1000 mL of the high-pH antigen retrieval buffer includes: 1.20 g of tris(hydroxymethyl) aminomethane (Tris), 0.35 g of ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), spit Warm-200.45mL, glycerol 300mL, ProClin 9500.4mL and the balance of purified water, the pH value range is pH8.9~9.1.

[0031] Preparation method: Weigh Tris and EDTA according to the proportion, dissolve Tris and EDTA with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClin950 after dissolution, and finally make up to volume with purified water. Rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the antigen retrieval buffer so that the pH value is in the range of pH 8.9 to 9.1.

Embodiment 3

[0033] A high pH antigen retrieval buffer: every 1000mL of the high pH antigen retrieval buffer includes: 1.25 g of tris(hydroxymethyl) aminomethane (Tris), 0.41 g of ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), spit Warm-200.6mL, glycerol 550mL, ProClin 9500.5mL and the balance of purified water, the pH value range is pH8.9 ~ 9.1.

[0034] Preparation method: Weigh Tris and EDTA according to the proportion, dissolve Tris and EDTA with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClin950 after dissolution, and finally make up to volume with purified water. Rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the antigen retrieval buffer so that the pH value is in the range of pH 8.9 to 9.1.

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Abstract

The invention discloses a high-pH antigen repair buffer solution adaptive to a full-automatic immunohistochemical staining machine and a preparation method of the high-pH antigen repair buffer solution. The high-pH antigen repair buffer solution is characterized by mainly comprising the following raw materials: tris (hydroxymethyl) aminomethane, ethylenediamine tetraacetic acid disodium salt dihydrate, tween-20, glycerol and ProClin950. The pH value of the repair buffer solution ranges from 8.9 to 9.1. According to the scheme, the growth of microorganisms in the in-vitro diagnostic reagent is effectively controlled, so that the antigen repair buffer solution can be stably stored and used for a long time under a cold storage condition or a room temperature condition, has better wettability, is not easy to volatilize at a relatively high temperature, avoids the drying condition of tissue slices, and improves the service life of the tissue slices. The pH value of the antibacterial preservative has the advantages of better stability and better antibacterial preservation function.

Description

technical field [0001] The application relates to the technical field of automatic immunohistochemical staining machines, in particular to a high pH (pH 8.9-9.1) antigen retrieval buffer adapted for automatic immunohistochemical staining machines and a preparation method thereof. [0002] technical background [0003] Immunohistochemical technology has developed rapidly in recent years, and with the application and promotion of automatic immunohistochemical staining instruments, the requirements for staining quality are also increasing. Among them, antigen retrieval plays an extremely important role in the entire immunohistochemical staining process, and the quality of the repair conditions directly determines the quality of the final staining results. Because of its own structural characteristics, the automatic staining instrument used to hold the repair solution is small in size, and cannot completely immerse tissue sections in the repair solution like manual repair. It ne...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/531G01N1/30
CPCG01N33/531G01N1/30
Inventor沈雯徐秀芬
Owner浙江莱阅病理诊断科技有限公司