Spleen regulating type B lymphocyte as well as preparation method and application thereof

A technology of B lymphocytes and spleen cells, applied in the field of cell biology, can solve problems such as glomerulonephritis and interstitial fibrosis that cannot be universally applied, and achieve good therapeutic potential, reduced thinning, and tubulointerstitial fibrosis lessening effect
CN114686427AActive Publication Date: 2022-07-01THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
Publication Date
2022-07-01

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Abstract

The invention provides a spleen regulating type B lymphocyte as well as a preparation method and application thereof. On one hand, the invention provides a spleen regulating type B lymphocyte, wherein the proportion of CD1d, CD5 and CD21 / 35 antibody positive regulating type B lymphocytes in the cell is more than 94.6%. The invention further provides a method for preparing the spleen regulating type B lymphocyte. The method for preparing the spleen regulating type B lymphocyte comprises the following steps: taking a spleen cell suspension, and marking with surface antibodies CD1d, CD5 and CD21 / 35; the labeled cells are sorted by fluorescence activated cell sorting. The third aspect of the invention provides application of the spleen regulating type B lymphocyte in preparation of a preparation for targeted colonization in bilateral kidneys. The fourth aspect of the invention provides application of the spleen regulating type B lymphocyte in preparation of a product for kidney protection.
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Description

technical field

[0001] The invention relates to a spleen-regulated B lymphocyte and a preparation method and application thereof, in particular to a fluorescence-activated cell sorting technique, which belongs to the field of cell biology. Background technique

[0002] In recent years, the role of B lymphocytes in fibrosis has gradually attracted attention. B lymphocyte depletion therapy has a potential regulatory effect on the composition of immune cell subsets and the expression of fibroblast phenotype, which may be one of the ways of its anti-fibrotic effect.

[0003] Considering the complexity of B lymphocyte subset phenotypes, B lymphocyte depletion therapy targeting a single surface antigen may not be universally applicable to all types of glomerulonephritis and its induced interstitial fibrosis. The reason why B lymphocyte depletion therapy targeting a single surface antigen, especially a single broad-spectrum surface antigen, is not applicable in some diseases and p...

Claims

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