Construction method of characteristic chromatogram of traditional Chinese medicine for clearing heat and eliminating phlegm and characteristic chromatogram thereof

Abstract

The invention discloses a construction method of a characteristic chromatogram of a traditional Chinese medicine for clearing heat and eliminating phlegm, and relates to the technical field of pharmaceutical analys.The construction method comprises the following steps that firstly, a reference substance solution is prepared, specifically, a proper amount of 6-methoxyguaiacol reference substance is prepared into a solution with the concentration of 0.03 mg / ml per 1 mL through methyl alcohol, and the reference substance solution is obtained; step 2, preparation of a test solution: precisely measuring 30ml of traditional Chinese medicine for clearing heat and eliminating phlegm, shaking and extracting with ethyl acetate for three times (30ml each time), combining ethyl acetate liquid, spin-drying at low temperature, adding methanol into residues for dissolving, and fixing the volume to 5ml; and step three, chromatographic condition determination: precisely sucking 1 microliter of the reference solution and 1 microliter of the test solution, injecting the solutions into a gas chromatograph, heating according to a specific mode, and recording a 36min chromatogram to obtain a GC characteristic spectrum of the traditional Chinese medicine for clearing heat and eliminating phlegm. The characteristic chromatogram can be used for comprehensively and integrally controlling and evaluating the quality, and the authenticity, integrity and consistency of the characteristic chromatogram are ensured.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing a characteristic map of a traditional Chinese medicine for clearing heat and resolving phlegm and the characteristic map thereof. Background technique [0002] The traditional Chinese medicine for clearing heat and resolving phlegm is the liquid naturally leached out of the fresh stems of several plants of the Poaceae genus, after being heated. , children with phlegm heat convulsions, etc., "Ben Cao Yan Yi" called "the holy potion of phlegm". From the database on the website of the State Drug Administration, there are a total of 43 single prescriptions and their compound preparations with the approval number of the traditional Chinese medicine preparation, of which 24 are for single preparations and 19 are for compound preparations, involving many productions nationwide. Enterprise, Jiangxi is the main producing area. [0003] The current quality...

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Problems solved by technology

However, there are more than 50 species of Bamboo genus, which are also affected by factors such as the place of origin, harvesting period, etc., and the processing method does not have specific regulations on its specific heating temperature and time parameters.

Therefore, affected by factors such as its origin, place of origin, harvesting period, processing method, etc., the current statutory quality standard for heat-clearing and phlegm-resolving traditional Chinese medicine only has traits, TLC identification, and pH value, total solids, and relative density inspection items, which are far from enough To fully reflect the pros and cons of its quality, and it is difficult to guarantee the authenticity, integrity and consistency of quality

[0004] It is reported in the literature that this traditional Chinese medicine mainly contains phenols, lignin and amino acids, and the phenolic components are its medicinal ingredients. At present, the quality control technical reports of this traditional Chinese medicine mainly focus on single-component phenols, sugars and amino acids. In terms of content determination of components, it is difficult to comprehensively control and evaluate the quality of TCM for clearing heat and resolving phlegm, and there are no related research reports on the characteristic map of TCM for clearing heat and resolving phlegm.

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