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Feeding additive for barbus hollandi fry and application of feeding additive in barbus hollandi fry cultivation

A Hastelloy formula and additive technology, applied in the field of aquaculture, can solve problems such as difficult to find traces

Active Publication Date: 2022-07-22
西双版纳云博水产养殖开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there has been almost no news of the discovery of wild catfish in the Lancang River, indicating that it is difficult to find its trace in the Lancang River.

Method used

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  • Feeding additive for barbus hollandi fry and application of feeding additive in barbus hollandi fry cultivation
  • Feeding additive for barbus hollandi fry and application of feeding additive in barbus hollandi fry cultivation
  • Feeding additive for barbus hollandi fry and application of feeding additive in barbus hollandi fry cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation of Culture Medium

[0032] The present embodiment utilizes the nutrient solution containing different nutrients to cultivate Paramecia, and the specific nutrient solution types involved and their corresponding preparation methods are as follows:

[0033] (1) Brassica napus extract culture solution: take fresh Brassica oleracea leaves, absorb the moisture on the surface of the leaves after cleaning, smash the background of the leaves and fully squeeze out the slurries of the cabbage leaves, filter the extruded slurries with gauze, and collect the filtrate, 15 g of the filtrate was added to 450 mL of water, boiled, cooled to room temperature, and allowed to stand for 1 day to obtain a culture solution for paramecia inoculation.

[0034] (2) Cooked egg yolk culture solution: mash 0.5 g of egg yolk, add 100 mL of cold boiled water to it, stir well to fully mix the egg yolk and water, and let stand for 1 day to obtain a culture solution for paramecium inoculat...

Embodiment 2

[0049] 1. Paramecia culture

[0050] The paramecia culture method adopted in the present embodiment is as follows:

[0051] Take fresh lettuce leaves, clean them, absorb the moisture on the surface of the leaves, smash the background of the leaves and fully squeeze out the pulp of the leaves, filter the extruded pulp with gauze, collect the filtrate, add 15g of the filtrate to 450mL of water, and boil. After cooling, 0.3 g of the freeze-dried powder of bacterial strain is added thereto, and the culture solution for inoculation of paramecium is obtained after the freeze-dried powder of the composite bacterial strain is completely dissolved. Use a capillary pipette to inoculate 20 paramecia into the container containing the above-mentioned culture solution, then cover the opening of the container with gauze, and cultivate the paramecia at a constant temperature of 25°C and pH=6.8±0.2. The culture period was 5 days, and one tenth of the culture medium in the culture vessel was p...

Embodiment 3

[0059] In this example, the paramecia culture system obtained after the culturing period of the seven treatment groups in Example 2 was used as the base liquid, and the influence of the strain regulator on the paramecia culture system was explored. In this example, the same base solution was used to divide into 4 treatment groups, which were specifically marked as treatment group 1, treatment group 2, treatment group 3 and treatment group 4. Bacillus, Escherichia coli and Paramecia were counted.

[0060] Treatment group 1: The ethanol extract of Rhizoma Rhizoma was added to the base solution, and the addition amount of the extract of Rhizoma Rhizoma was 8 wt% of the base solution, and the culture was continued for 8 hours.

[0061] Treatment group 2: The ethanol extract of Rhizoma Imperatae was added to the base solution, and the amount of the extract of Rhizoma Rhizoma was 8 wt% of the base solution, and the culture was continued for 8 hours.

[0062] Treatment 3 groups: the...

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Abstract

A preparation method of the feeding additive comprises the following steps: preparing a culture solution, adding a leaf lettuce extract into purified water, boiling and cooling; constructing a paramecium culture system, and inoculating paramecium into a culture solution to form the paramecium culture system; and culturing paramecium at 20-28 DEG C for 5-7 days. According to the method, the culture solution containing the leaf lettuce extract is used for culturing the paramecium, the growth form of the paramecium can be regulated and controlled, the paramecium obtained through culture is in a long and thin shape and small in size, the feeding preference of the spinibarbus hollandi larvae is met, exogenous feeding of the spinibarbus hollandi larvae is promoted, and the spinibarbus hollandi larvae are promoted. And the survival rate of the spinibarbus hollandi larvae is improved. On the other hand, the paramecia has good proliferation activity in the leaf lettuce extract culture solution, a paramecia community with large density can be formed in the leaf lettuce extract culture solution, and the paramecia community can serve as a rich nutrition source of the spinibarbus hollandi fry.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a feeding additive for the larvae of Hastelloy's square mouth and its application in the cultivation of the larvae of the Haste's square mouth. Background technique [0002] As one of the rare and endangered indigenous fishes in the Lancang River, the Ha's square-mouthed barb belongs to the genus Cyprinidae, and generally inhabits the middle to deep bottom waters of the middle and lower reaches of the Mekong River. In recent years, there has been almost no news of the discovery of the wild Ha's squaremouth in the Lancang River, indicating that it has been difficult to find its traces in the Lancang River. Therefore, it is of great practical significance to develop the artificial breeding technology of Hashi's square mouth barb, so that the Ha's square mouth barb can get rid of the fate of extinction. SUMMARY OF THE INVENTION [0003] The purpose of the present invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K10/30A23K20/00A01K61/10C12N1/20C12N1/16C12N1/10C12R1/19C12R1/125C12R1/225C12R1/645C12R1/90
CPCA23K50/80A23K10/18A23K10/30A23K20/00A01K61/10C12N1/20C12N1/16C12N1/10Y02A40/81
Inventor 王云清王敏奇曹建萌朱峥恺李燕李怡凌石凤德黄绍刚普忠南
Owner 西双版纳云博水产养殖开发有限公司
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