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Preparation method of proportion mixture of adipose-derived mesenchymal stem cells and nano fat

A technology of mesenchymal stem cells and mixtures, which is applied in the field of preparation of mixtures of adipose-derived mesenchymal stem cells combined with nano-fat, which can solve the problems of large side effects and achieve a good repairing effect

Pending Publication Date: 2022-07-29
高山艳
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Clinically, steroid hormones are often used in combination with topical minoxidil, but the side effects are large, and recurrence will occur after stopping the drug

Method used

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  • Preparation method of proportion mixture of adipose-derived mesenchymal stem cells and nano fat
  • Preparation method of proportion mixture of adipose-derived mesenchymal stem cells and nano fat
  • Preparation method of proportion mixture of adipose-derived mesenchymal stem cells and nano fat

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Embodiment Construction

[0034] Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure will be more thoroughly understood, and will fully convey the scope of the present disclosure to those skilled in the art.

[0035] It is to be understood that the terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting. As used herein, the singular forms "a," "an," and "the" can also be intended to include the plural forms unless the context clearly dictates otherwise. The terms "comprising", "comprising", "containing" and "having" are inclusive and thus indicate the presenc...

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Abstract

The invention provides a preparation method of a proportional mixture of adipose-derived mesenchymal stem cells and nano-fat. The method comprises the following steps: S1, separating and extracting adipose-derived mesenchymal stem cells; s2, culturing the adipose tissue-derived stem cells; s3, screening the adipose-derived mesenchymal stem cells cultured in the step S2; s4, preparing nano fat; and S5, mixing the nano fat and the screened adipose-derived mesenchymal stem cells to obtain a mixture with a required proportion. The proportion mixture prepared by the preparation method of the proportion mixture of the adipose-derived mesenchymal stem cells and the nano-adipose provided by the invention has the advantages that the proportion mixture is uniform; the stem cells exist in the nano fat and can promote collagen synthesis of fiber cells and angiogenesis of vascular endothelial cells, and the bioactive factors are secreted by the adipose-derived stem cells, and the adipose-derived stem cells can continuously activate and nourish the skin hair follicle structure to promote functional change of the skin hair follicle structure. The effect of repairing damaged skin and damaged hair follicles can be better achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a proportional mixture of adipose mesenchymal stem cells and nano-fat. Background technique [0002] The skin is a very important barrier for the human body, which can not only prevent the loss of water, electrolytes and other nutrients in the body, but also prevent the invasion of external harmful substances. In addition, the skin also directly affects the appearance of a person. It can be seen that the health of the skin is very important to the human body. Hair follicles play an important role in skin function. [0003] The hair follicles in the skin are composed of epithelial cells and mesenchymal cells, contain a large number of stem cells, and are attached to each other by surrounding glands. The structure is complex, and once damaged, it is difficult to repair. [0004] With the improvement of living standards and the faster pace of life, ...

Claims

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Application Information

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IPC IPC(8): A61K35/35A61K35/28A61K47/46A61P17/00A61P17/14C12N5/0775
CPCA61K35/28A61K35/35A61K47/46A61K9/0019A61P17/14A61P17/00C12N5/0667C12N2509/10A61K2300/00
Inventor 高山艳
Owner 高山艳
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