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Pseudomonas mendocina capable of effectively degrading atrazine and application of Pseudomonas mendocina

A technology of atrazine and monas, which is applied in the field of bioremediation to achieve good repair effect and obvious effect of promoting growth

Active Publication Date: 2020-04-28
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is reported that Pseudomonas mendoza can degrade pesticides such as methamidophos, fomesafen, and monoformamidine, but there is no report on the degradation of atrazine

Method used

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  • Pseudomonas mendocina capable of effectively degrading atrazine and application of Pseudomonas mendocina
  • Pseudomonas mendocina capable of effectively degrading atrazine and application of Pseudomonas mendocina
  • Pseudomonas mendocina capable of effectively degrading atrazine and application of Pseudomonas mendocina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of atrazine-degrading bacteria

[0030] Screening method: Collect soil in the cornfield of Yuanyang Experimental Base of Henan Academy of Agricultural Sciences, take 10g of soil sample, add it to the basal salt medium (100mL) containing atrazine 10mg / L, mix well, and heat at 30°C , 180r / min shaking culture; every 7d, transfer the culture to the basal salt medium with increasing concentration of arazine at a transfer rate of 10%, and the concentration of alatazine in the basal salt medium is 10mg / L and 20mg respectively / L, 30mg / L, 50mg / L, 100mg / L; when the concentration of atrazine was 100mg / L, the enrichment culture was diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Five concentrations, 100 μL of each concentration were spread on the solid basic salt culture plate containing atrazine 100mg / L, and the plate was placed in a 30°C incubator for culture. After 3-5 days of culture, pick out the hydrolysis circle The colonies were isolated and purified...

Embodiment 2

[0032] Example 2: Degradation Ability Determination of Strain SFAD3

[0033] Pick a single colony of SFAD3 and inoculate it in LB liquid medium, culture at 30°C with shaking at 200r / min for 16-20 hours; take the cultured bacteria liquid overnight, centrifuge at 4000r / min for 10min, discard the supernatant, and wash 3 times with basic salt medium ; Precipitate with basal salt medium prepared to 1 × 10 9 cfu / mL bacterial suspension. The bacterial suspension was added to the basal salt medium containing 100mg / L atrazine at an inoculated amount of 2%, cultured with shaking at 30°C and 180r / min, and no inoculation was set as the control, with 3 replicates each. Samples were taken when culturing 3d, 6d, 9d, 12d, and 15d respectively, extracted 3 times with dichloromethane, and the volume of dichloromethane was 50%, 20%, and 20% of the sample volume in turn, the extract was evaporated to dryness, and methanol ( High performance liquid chromatography grade) was dissolved, and the vo...

Embodiment 3

[0038] Example 3: Morphological observation and physiological and biochemical identification of bacterial strain SFAD3

[0039] The colony morphology of SFAD3 grown on the LB plate was observed, and the characteristics of the bacteria were observed by Gram staining; the physiological and biochemical indicators of the strain were determined according to "Berger's Bacterial Identification Manual" (Eighth Edition).

[0040] The morphological observation of the strain SFAD3 showed that the colonies of SFAD3 on the LB medium were viscous, shiny, opaque, with smooth surfaces, irregular edges, light yellow colonies, and no pigmentation; Gram staining was negative, no spores, and the cells were short. Rod-shaped; the physiological and biochemical assay results of SFAD3 are shown in Table 2, methyl red reaction, gelatin hydrolysis test, starch hydrolysis test, chitin hydrolysis test, cellulose hydrolysis test and tyrosine hydrolysis test are all negative, peroxidation The hydrogenase t...

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Abstract

The invention relates to Pseudomonas mendocina capable of effectively degrading atrazine and application of the Pseudomonas mendocina, and belongs to the technical field of bioremediation. The Pseudomonas mendocina SFAD3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2019302. The invention also provides a screening method of the strain and abacterial suspension obtained by applying the strain. The Pseudomonas mendocina SFAD3 disclosed by the invention is cultured for 15 days in a basic salt culture medium added with 100mg / L atrazine, and the degradation rate reaches 50.06%, which indicates that the strain can effectively degrade the atrazine; after the SFAD3 is treated in soil containing the atrazine for 30 days, the degradation rate reaches 72.6%, which indicates that the SFAD3 has a relatively good remediation effect on the soil polluted by the atrazine; and meanwhile, Pseudomonas mendocina fermentation broth screened by the method has a certain promoting effect on the growth of sesame seedlings.

Description

technical field [0001] The invention relates to a Pseudomonas mendoza strain capable of effectively degrading atrazine, belonging to the technical field of bioremediation. Background technique [0002] Atrazine (atrazine) common name atrazine, chemical name is 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, belongs to triazine herbicides, widely used It is used to prevent and control annual grass weeds crabgrass, barnyardgrass, foxtail, sedge, cruciferous and leguminous weeds in upland crops such as corn, sorghum and soybean, and also has a certain inhibitory effect on perennial weeds. Atrazine is a low-toxic herbicide, because of its long persistence and half-life in the soil, it is easy to cause phytotoxicity to sensitive crops such as rice, wheat, and soybean; it pollutes groundwater and surface water, threatening human and animal health and ecological environment. [0003] Microbial degradation is a well-recognized method for the removal of pollutants in an envi...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01N63/27A01P21/00C12R1/38
CPCC12N1/20B09C1/10A01N63/00C12R2001/38C12N1/205
Inventor 倪云霞何碧珀刘新涛赵辉刘红彦赵新贝千慧敏
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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