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Single spore separation device for phytophthora parasitica var nicotianae

A technology for the isolation of blackleg bacteria and single spores, which is applied in biochemical cleaning devices, enzymology/microbiology devices, sterilization methods, etc., can solve the problems of poor separation effect, reduce the transportation process, ensure safety, Easy to Categorize Effects

Inactive Publication Date: 2022-08-02
CROP INST ANHUI PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A single spore separation device for rapeseed black shank bacteria proposed by the invention solves the problem of poor separation effect

Method used

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  • Single spore separation device for phytophthora parasitica var nicotianae
  • Single spore separation device for phytophthora parasitica var nicotianae
  • Single spore separation device for phytophthora parasitica var nicotianae

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Embodiment Construction

[0057] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments.

[0058] refer to Figure 1-10 , a single spore separation device of blackleg bacterium of rape, comprising:

[0059] Bottom box 1, the top of which is fixedly connected with execution box 2, one side of the execution box 2 is rotatably connected with a sealing door 3, the sealing door 3 is connected with an adding mechanism, one side of the bottom box 1 is connected with a discharge door 69, and the bottom box 1. Carry out necessary support and placement to ensure the stability of the entire work. The adding mechanism is convenient for adding to the execution box 2. The discharge door 69 can discharge the culture dish on the belt 70.

[006...

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Abstract

The invention discloses a single spore separation device for phytophthora parasitica var nicotianae, relates to the technical field of strain culture, and provides the following scheme aiming at the problem of poor separation effect in the prior art: the single spore separation device for phytophthora parasitica var nicotianae comprises a bottom box, and the top of the bottom box is fixedly connected with an execution box; the top of the limiting ring is rotationally connected with a rotating plate, and the rotating plate is connected with an auxiliary mechanism; a sampling mechanism is placed on the limiting frame, and one side of the sampling mechanism is connected with a disinfection mechanism located in the execution box; the rubber gloves are fixedly connected to the execution box, and a microscope connected with the execution box is arranged between the two rubber gloves. The device is simple in structure and convenient to use, diversified operation can be carried out in the execution box, meanwhile, environmental factors such as internal temperature are controlled, bacterial colonies are well cultured, and then in a sealed environment, the operation is simple, and the operation is convenient. Dilution and stabilization are carried out, and overall separation work is guaranteed.

Description

technical field [0001] The invention relates to the technical field of bacterial strain culture, in particular to a device for separating single spores of black shank bacteria of rapeseed. Background technique [0002] Rapeseed blackleg is a widespread fungal disease of rape and other Brassica plants worldwide. In popular years, rapeseed yields can be reduced by 20%-60%. The pathogens that cause this disease are two closely related species of the genus Paleobacter, one is Paleococcus maculatum and the other is Paleococcus diglobosa. [0003] At present, L. maculans has not been found in my country. In 1999, the pathogen L.biglobosa was identified from rapeseed. Subsequently, rapeseed blackleg was found in 14 provinces of rapeseed producing areas in winter and spring in my country. The incidence of black shank in the field varies from sporadic to 92%. In a small number of fields with severe disease, there are even up to 5% of the diseased dead plants, so in the actual res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/12C12M1/38
CPCC12M33/04C12M41/24C12M37/00
Inventor 初明光荣松柏李强生
Owner CROP INST ANHUI PROV ACAD OF AGRI SCI
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